Anti-Human GPR55 (extracellular) Antibody

G-protein coupled receptor 55
  • New
Cat #: ACR-061
Alternative Name G-protein coupled receptor 55
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    Immunogen
    • Peptide (C)HGKVEKYMSFHNMSD, corresponding to amino acid residues 160-174 of human GPR55 (Accession Q9Y2T6). 2nd extracellular loop.
    • Anti-Human GPR55 (extracellular) Antibody
    Accession (Uniprot) Number Q9Y2T6
    Gene ID 9290
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Specificity The antibody will not recognize the protein from rat or mouse samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Human GPR55 (extracellular) Antibody
      Western blot analysis of human NK-92MI natural killer cell (lanes 1 and 3) (1:500) and human acute monocytic leukemia THP-1 cell (lanes 2 and 4) (1:200) lysates:
      1, 2. Anti-Human GPR55 (extracellular) Antibody (#ACR-061).
      3, 4. Anti-Human GPR55 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Human GPR55 (extracellular) Antibody
      Western blot analysis of human ovary adenocarcinoma OVCAR-3 cell (lanes 5 and 7) and human pancreatic carcinoma PANC-1 cell (lanes 6 and 8) lysates:
      5, 6. Anti-Human GPR55 (extracellular) Antibody (#ACR-061), (1:1000).
      7, 8. Anti-Human GPR55 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Human GPR55 (extracellular) Antibody
      Western blot analysis of human HMC3 microglia cell (lanes 9 and 11) (1:500) and human chronic myelogenous leukemia MEG-O1 cell (lanes 10 and 12) (1:200) lysates:
      9, 10. Anti-Human GPR55 (extracellular) Antibody, (#ACR-061).
      11, 12. Anti-Human GPR55 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Human GPR55 (extracellular) Antibody
      Cell surface detection of GPR55 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Human GPR55 (extracellular) Antibody (#ACR-061), (2.5 μg/5x105 cells) + goat-anti-rabbit-FITC.
    References
    1. Sharir, H. and Abood, M.E. (2010)Pharmacol. Ther. 126,301.
    2. Yang, H. et al. (2016) J. Basic Clin. Physiol. Pharmacol. 27, 297.
    3. Pertwee, R.G. et al. (2010) Pharmacol. Rev. 62, 588
    4. Di Marzo, V. (2009) Pharmacol. Res. 60, 77.
    5. Battista, N. et al. (2012) Front. Behav. Neurosci. 6, 9.
    Scientific background

    GPR55 is an endocannabinoid receptor that belongs to group δ of the rhodopsin-like (class A) receptors. Like all other G-protein coupled receptors, it consists of seven trans-membrane helixes, intracellular C-terminal domain and extracellular N-terminal extension1.

    The other known cannabinoid receptors, CB1 and CB2 share low homology with GPR55 and have different ligand specificity and different downstream activated cascade. Thus, GPR55 consists on its own a class of cannabinoid receptor2.

    GPR55 is highly expressed in the striatum and binds a wide range of ligands, among which are endocannabinoids:  Anadanine and 2-Arachidonoyl Glycerol, and phytocannabinoids: Tetrahydrocannabinol (THC), abnormal cannabinoid (CBD) and cannabidiol3. Following ligand binding, GPR55 couples to Gα12,13 proteins, which eventually lead to increased intracellular Ca2+ levels2.

    The endocannabinoid system has a tremendous potential for experimental and clinical research, based on their evolutionary conservation and role in fundamental physiological and pathological processes, including pain modulation, immune function, neuroprotection, cancer, cardiovascular diseases, fertility and appetite4. Clinical studies using cannabinoid receptor agonists and antagonists have suggested their possible use in modulating acute and/or chronic diseases such as hypertension, glaucoma, emesis, anxiety, depression, gastrointestinal and hepatic disorders, ulcerative colitis and neuropathic and inflammatory pain5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 27/07/2020

    Anti-Human GPR55 (extracellular) Antibody (#ACR-061) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human samples only.

    For research purposes only, not for human use
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