Anti-GPR68 (OGR1) (extracellular) Antibody

Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor
    Cat #: AGR-042
    Alternative Name Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68 (Accession Q8BFQ3). 2nd extracellular loop.
    • Anti-GPR68 (OGR1) (extracellular) Antibody
    Accession (Uniprot) Number Q8BFQ3
    Gene ID 238377
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, human – 14/15 amino acid residues identical.
    RRID AB_2756632.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-GPR68 (OGR1) (extracellular) Antibody
      Western blot analysis of rat kidney lysates (lanes 1 and 3) and mouse kidney membranes (lanes 2 and 4):
      1,2. Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (1:200).
      3,4. Anti-GPR68 (OGR1) (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-GPR68 (OGR1) (extracellular) Antibody
      Western blot analysis of rat heart membranes:
      1. Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (1:200).
      2. Anti-GPR68 (OGR1) (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-GPR68 (OGR1) (extracellular) Antibody
      Cell surface detection of GPR68 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (2.5 µg) + goat-anti-rabbit-FITC.
    1. Thompson, M.D. et al. (2005) Crit. Rev. Clin. Lab Sci. 42, 311.
    2. Xu, Y. and Casey, G. (1996) Genomics. 35, 397.
    3. Zaslavsky, A. et al. (2006) Biochim. Biophys. Acta. 1761, 1200.
    4. Mohebbi, N. et al. (2012) Cell. Physiol. Biochem. 29, 313.
    Scientific background

    All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR42.

    GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/07/2020

    Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples.

    For research purposes only, not for human use
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