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Home › Products › G-Protein Coupled Receptors › Cannabinoid Receptors › Antibodies

Certificate of Analysis

Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody

CB2, CNR2

Cat #: ACR-003
Alternative Name CB2, CNR2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human CB2 receptor (Accession P34972). Extracellular, N-terminus.
Accession (Uniprot) Number P34972
Gene ID 1269
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Canis, bovine - 10/14 amino acid residues identical.
RRID AB_2039797.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended to use with rat and mouse samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, lci, wb
May also work in: ih*, ip*
Western blot
  • HL-60 (human promyelocytic leukaemia) and MCF-7 (human adenocarcinoma, mammary gland) cell line lysates (1:200).
  • Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysate (lanes 1 and 2) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 4): 
    Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysate (lanes 1 and 2) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 4): 
    1,3. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody  (#ACR-003), (1:200).
    2,4. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody, preincubated with Human Cannabinoid Receptor 2 (extracellular) Blocking Peptide (#BLP-CR003).
Immunocytochemistry
  • Human prostate cancer (LNCaP) live cells (1:50).
Indirect flow cytometry
  • Human promyelocytic leukaemia live cells (5 μg).
Scientific background

Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3

The CB2 is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6

Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.7-9 In human astrocytoma a direct relation between CB2 expression and tumor malignancy was demonstrated.8 Activation of CB2R in vivo by its agonist JWH-133, completely blocked cell growth.8 In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
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Last update: 08/01/2023

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For research purposes only, not for human use

Specifications

Citations

Citations

Applications

Scientific Background

Shipping and Ordering information
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    Control Product

    • Human Cannabinoid Receptor 2 (extracellular) Blocking Peptide (#BLP-CR003) is the original antigen used for immunization during Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (#ACR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Human Cannabinoid Receptor 2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

      Human Cannabinoid Receptor 2 (extracellular) Blocking Peptide (#BLP-CR003)

    Resources

    • Cannabinoid Signaling in the Nervous System

    General Protocols

    • Blocking Peptides – Controls for better results
    • Blocking Peptide Protocol for Western Blot (WB)
    • Sample Preparation for Cell Lines
    • Immunocytochemistry (ICC) Protocols for Fixed or Live Cells: Indirect and Direct Methods
    • Flow Cytometry Protocols for Live Cells: Indirect and Direct Methods
    • Western Blot (WB) Protocol

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    Resources

    • Cannabinoid Signaling in the Nervous System

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