Anti-Human CCR2 (extracellular) Antibody

C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192
  • New
Cat #: ACR-022
Alternative Name C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    Immunogen
    • Peptide RNTNESGEEVTTFFD(C), corresponding to amino acid residues 11-25 of human CCR2 (Accession P41597). Extracellular, N-terminus.
    • Anti-Human CCR2 (extracellular) Antibody
    Accession (Uniprot) Number P41597
    Gene ID 729230
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody will not recognize CCR2 from mouse and rat samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Human CCR2 (extracellular) Antibody
      Western blot analysis of human acute monocytic leukemia THP-1 (lanes 1 and 3) and human promyelocytic leukemia HL-60 (lanes 2 and 4) cell line lysates:
      1, 2. Anti-Human CCR2 (extracellular) Antibody (#ACR-022), (1:1500).
      3, 4. Anti-Human CCR2 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Human CCR2 (extracellular) Antibody
      Cell surface detection of CCR2 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Human CCR2 (extracellular) Antibody (#ACR-022) (2.5 μg/5x105 cells) + goat-anti-rabbit-FITC.
    References
    1. Zheng, Y. et al. (2016) Nature 540, 458.
    2. Fantuzzi, L. et al. (2019) Cell. Mol. Life Sci. 76, 4869.
    3. Taylor, B.C. et al. (2019) Proc. Natl. Acad. Sci. U.S.A. 116, 8131.
    Scientific background

    CC chemokine receptor 2 (CCR2) is one of 19 members of the chemokine receptor subfamily of class A G-protein-coupled receptors (GPCRs). 

    CCR2 is expressed in monocytes, immature dendritic cells, and T-cell subpopulations, and mediates acute inflammation by driving leukocyte migration to damaged or infected tissues towards chemokine ligands such as CCL2. Elevated expression of chemokines and their receptors such as CCR2 and its ligands can contribute to chronic inflammation and malignancy and hence these molecules are implicated in numerous inflammatory and neurodegenerative diseases including atherosclerosis, multiple sclerosis, asthma, neuropathic pain, and diabetic nephropathy, as well as cancer. These disease associations have motivated numerous studies in search of therapies that target the CCR2-chemokine axis1,2.

    As with most G-protein coupled receptors (GPCRs), chemokine receptors transmit signals across cell membranes by means of extracellular ligand and intracellular G-protein binding. CCR2 has seven transmembrane alpha helixes, and can take distinct conformational states that are necessary for chemokine/ligand binding, G-protein binding, activation, inactivation, and signal transmission3.

    CCR2 can form homo- or heterodimers with other chemokine receptors. Homodimerization may be necessary for CCR2 chemotactic activity and occurs in the absence of the ligand. CCR2/CCR5 heterocomplexes activate calcium response and support cell adhesion rather than chemotaxis, whereas CCR2/CCR4 heterodimers have an allosteric trans-inhibitory effect on CCL2 binding2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 24/01/2020

    Anti-Human CCR2 (extracellular) Antibody (#ACR-022) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, indirect live cell flow cytometry applications. It has been designed to recognize CCR2 from human samples only. The antibody will not recognize CCR2 from mouse and rat samples.

    For research purposes only, not for human use
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