Anti-CCR9 (extracellular) Antibody

C-C chemokine receptor type 9, G-protein coupled receptor 28 GPR-9-6, CDw199
  • New
Cat #: ACR-029
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DESTKLKSAVLTLK, corresponding to amino acid residues 205-218 of human CCR9 (Accession P51686). 2nd extracellular loop.
    Accession (Uniprot) Number P51686
    Gene ID 10803
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – 10/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic, ih, lci
    Western blot
    Western blot analysis of human acute T cell leukemia (Jurkat) (lanes 1 and 3), human melanoma (MALME-3M) (lanes 2 and 4), human Adenocarcinoma (HT-29) (lanes 5 and 7) and mouse T-cell lymphoma (TK-1) (lanes 6 and 8) cell line lysates:
    1, 2, 5, 6. Anti-CCR9 (extracellular) Antibody (#ACR-029), (1:200).
    3, 4, 7, 8. Anti-CCR9 (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry of live intact rat lymphoma, T-lymphocyte (C58) cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-CCR9 (extracellular) Antibody (#ACR-029), (5 μg/0.5x106 cells) + goat anti-rabbit-FITC.
    References
    1. Arimont, M. et al. (2017) J. Med. Chem. 60, 4735.
    2. Tu, Z. et al. (2016) J. Hematol. Oncol. 9, 10.
    Scientific background

    CCR9 (C-C chemokine receptor type 9) belongs to the β-chemokine receptor family of the G-protein coupled receptor superfamily. Chemokine receptors play a crucial role in cell migration and are considered to be important therapeutic targets for inflammatory diseases and in tumor chemoresistance and metastasis. In addition, high expression of CCR9 has been reported in a wide range of cancers including melanoma, ovarian cancer, lung cancer, breast cancer and colon cancer. Thus, CCR9 is considered to be a potential tumor biomarker in diagnosis and therapy of cancer1,2. Under physiological conditions, CCR9 is mainly expressed in immature T lymphocytes and on the surface of intestinal cells2.

    CCR9 receptor interacts with its specific ligand- chemokine ligand 25 (CCL25, thymus-expressed chemokine) in a two-step binding mechanism in which the C-terminal region of CCL25 first binds the N-terminus region and extracellular loops of the receptor followed by the binding of the N-terminus of the chemokine. The two-step biding mechanism stabilizes the receptor in an active conformation that facilitates intracellular signaling1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 20/09/2018

    Anti-CCR9 (extracellular) Antibody (#ACR-029) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, indirect live cell flow cytometry applications. It has been designed to recognize CCR9 from rat, mouse, and human samples.

    For research purposes only, not for human use