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Anti-Human CCR3 (extracellular) Antibody

C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen

Cat #: ACR-023
Alternative Name C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide CSALYPEDTVYSWRH, corresponding to amino acid residues 183-197 of human CCR3 (Accession P51677). 2nd Extracellular loop.
Accession (Uniprot) Number P51677
Gene ID 1232
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with rat and mouse samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of human malignant melanoma cell line Malme-3M (lanes 1 and 3) and human monocytic leukemia THP-1 cell line (lanes 2 and 4) lysates:
    Western blot analysis of human malignant melanoma cell line Malme-3M (lanes 1 and 3) and human monocytic leukemia THP-1 cell line (lanes 2 and 4) lysates:
    1,2. Anti-Human CCR3 (extracellular) Antibody (#ACR-023), (1:200).
    3,4. Anti-Human CCR3 (extracellular) Antibody, preincubated with Human CCR3 (extracellular) Blocking Peptide (#BLP-CR023).
Indirect flow cytometry
  • Cell surface detection of CCR3 in live intact human Jurkat T cell leukemia cells:
    Cell surface detection of CCR3 in live intact human Jurkat T cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-Alexa-488.
    ___ Cells + Anti-Human CCR3 (extracellular) Antibody (#ACR-023), (5 µg/1x106 cells) + goat-anti-rabbit-Alexa-488.
  • The blocking peptide is not suitable for this application.
References
  1. Zhu, X. et al. (2018) Mol. Med. Rep. 18, 3780.
  2. Zhang, J. et al. (2016) eNeuro 3, 5.
Scientific background

CCR3 (C-C chemokine receptor type 3) is a seven transmembrane G-protein coupled receptor (GPCR) that acts as a key regulator in the homeostasis of the immune system. The receptor is responsible for recruiting eosinophils to local inflammatory tissues.

CCR3 is the endogenous receptor for Eotaxin, C-C motif chemokine 11 (CCL11). The binding between CCR3 and CCL11 activates G-protein-dependent signaling pathways and thus enables the release of eosinophils progenitor cells and mature eosinophils to the affected tissue. Studies have shown that deficiency in CCR3 protein may reduce the binding of Eotaxin and inhibition of phosphatidylinositol-3-kinase (PI3K) signaling pathway activation and the inactivation of its subunit PI3Kγ1. Other CCR3 ligands include CCL5, CCL24, CCL26, CCL8, CCL7, and CCL121.

CCR3 is linked to several type-I allergy diseases, including allergic rhinitis. In addition to CCR3 expression in eosinophils, CCR3 is also expressed in basophils, microglia, astrocytes and neurons in both the developing and adult brain1,2.

The expression of CCR3 increases following human immunodeficiency virus infection and in reactive microglia and astrocytes around the amyloid deposits in Alzheimer’s disease2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Anti-Human CCR3 (extracellular) Antibody (#ACR-023) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CCR3 from human samples only. The antibody will not recognize the receptor from mouse or rat samples.

For research purposes only, not for human use
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