Anti-Human CCR3 (extracellular) Antibody

C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen
    Cat #: ACR-023
    Alternative Name C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    • Peptide CSALYPEDTVYSWRH, corresponding to amino acid residues 183-197 of human CCR3 (Accession P51677). 2nd Extracellular loop.
    • Anti-Human CCR3 (extracellular) Antibody
    Accession (Uniprot) Number P51677
    Gene ID 1232
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for use with rat and mouse samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 μl PBS.
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-Human CCR3 (extracellular) Antibody
      Western blot analysis of human malignant melanoma cell line Malme-3M (lanes 1 and 3) and human monocytic leukemia THP-1 cell line (lanes 2 and 4) lysates:
      1,2. Anti-Human CCR3 (extracellular) Antibody (#ACR-023), (1:200).
      3,4. Anti-Human CCR3 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-Human CCR3 (extracellular) Antibody
      Cell surface detection of CCR3 in live intact human Jurkat T cell leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-Alexa-488.
      ___ Cells + Anti-Human CCR3 (extracellular) Antibody (#ACR-023), (5 µg/1x106 cells) + goat-anti-rabbit-Alexa-488.
    • The negative control antigen is not suitable for this application.
    1. Zhu, X. et al. (2018) Mol. Med. Rep. 18, 3780.
    2. Zhang, J. et al. (2016) eNeuro 3, 5.
    Scientific background

    CCR3 (C-C chemokine receptor type 3) is a seven transmembrane G-protein coupled receptor (GPCR) that acts as a key regulator in the homeostasis of the immune system. The receptor is responsible for recruiting eosinophils to local inflammatory tissues.

    CCR3 is the endogenous receptor for Eotaxin, C-C motif chemokine 11 (CCL11). The binding between CCR3 and CCL11 activates G-protein-dependent signaling pathways and thus enables the release of eosinophils progenitor cells and mature eosinophils to the affected tissue. Studies have shown that deficiency in CCR3 protein may reduce the binding of Eotaxin and inhibition of phosphatidylinositol-3-kinase (PI3K) signaling pathway activation and the inactivation of its subunit PI3Kγ1. Other CCR3 ligands include CCL5, CCL24, CCL26, CCL8, CCL7, and CCL121.

    CCR3 is linked to several type-I allergy diseases, including allergic rhinitis. In addition to CCR3 expression in eosinophils, CCR3 is also expressed in basophils, microglia, astrocytes and neurons in both the developing and adult brain1,2.

    The expression of CCR3 increases following human immunodeficiency virus infection and in reactive microglia and astrocytes around the amyloid deposits in Alzheimer’s disease2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 19/02/2020

    Anti-Human CCR3 (extracellular) Antibody (#ACR-023) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CCR3 from human samples only. The antibody will not recognize the receptor from mouse or rat samples.

    For research purposes only, not for human use
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