- Peptide (C)ESVTENFEYDDLAE, corresponding to amino acid residues 6-19 of human CX3CR1 (Accession P49238). Extracellular, N-terminus.
- Human NK-92MI natural killer cell line and human THP-1 acute monocytic leukemia cell line (1:600-1:3000).
- Western blot analysis of human NK-92MI natural killer cells (lanes 1 and 3) and human THP-1 acute monocytic leukemia (lanes 2 and 4) cell lysates:1,2. Anti-Human CX3CR1 (extracellular) Antibody (1:600), (#ACR-059).
3,4. Anti-Human CX3CR1 (extracellular) Antibody, preincubated with Human CX3CR1 (extracellular) Blocking Peptide (#BLP-CR059).
- Human THP-1 monocytic leukemia cells (2.5 µg/5x105 cells).
CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.
CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).
Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.