- Peptide (C)DVHLFDYVEPGNYSD, corresponding to amino acid residues 2-16 of rat CXCR7 (ACKR3) (Accession O89039). Extracellular, N-terminus.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-CXCR7 (ACKR3) (extracellular) Antibody (#ACR-037), (1:400).
3,4. Anti-CXCR7 (ACKR3) (extracellular) Antibody, preincubated with CXCR7/ACKR3 (extracellular) Blocking Peptide (#BLP-CR037).
- Cell surface detection of CXCR7 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-CXCR7 (ACKR3) (extracellular) Antibody (#ACR-037), (2.5μg) + goat-anti-rabbit-FITC.
- Griffith, J.W. et al. (2014) Annu. Rev. Immuno. 32, 659.
- Sun, X. et al. (2010), Cancer Metastasis Rev. 29, 709.
- Burns, J.M. et al. (2006) J. Exp. Med. 203, 2201.
- Jones, S.W. et al. (2006) Osteoarthritis and Cartilage 14, 597.
- Miao, Z. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 15735.
- Boldajipour, B. et al. (2008) Cell 132, 463.
Chemokines are molecules that control the migration and positioning of the immune system cells. In addition to their function as chemo-attractants they have various other roles in the maturation of immune cells such as T-cell development in the thymus and homeostatic retention of immune cells in the bone marrow1.
Chemokines bind to chemokine receptors, members of the G-protein coupled receptor superfamily. They are comprised of seven transmembrane receptors with extracellular N-terminus, three extracellular and three intracellular loops and an intracellular C-terminus. One of the intracellular domains of the receptor couples with G proteins and begins a cascade of signal transduction2.
Until recently the CXCR-7 was an “orphan” receptor with its endogenous ligand unknown. This receptor is encoded by the CXCR7/RDC-1 gene which was mapped to mouse chromosome 1 and human chromosome 2. Homology has been shown with the viral gene ORF74 suggesting the receptor might signal without the binding of a ligand. Currently it is known that the chemokines CXCL12 and CXCL11 bind to CXCR-73.
CXCR-7 is tightly linked to the regulation of B cell development and differentiation indicating it might have a role in the formation of B memory cells4. CXCR-7 was also found to be associated with tumors where its levels are elevated in many tumor cell lines such as endothelial cells, fetal liver cells and placenta cells5. Despite this suggested role, there is growing evidence that this receptor does not function as a regular GPCR6.
Species reactivity key:
Anti-CXCR7 (ACKR3) (extracellular) Antibody (#ACR-037) is a highly specific antibody directed against an epitope of the rat CXCR7 chemokine receptor. The antibody can be used in western blot analysis and indirect flow cytometry. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR7 from human, rat and mouse samples.