Anti-Kir4.1 (KCNJ10) (extracellular) Antibody

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel Inwardly rectifying subfamily J member 10
    Cat #: APC-165
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)HGDLLELGPPANHT, corresponding to amino acid residues 93-106 of rat Kir4.1 (Accession P49655). Extracellular loop.
    Accession (Uniprot) Number P49655
    Gene ID 29718
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity May have some cross-reactivity with rat Kir4.2 (Accession Q91ZF1).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1, 2. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:500).
    3, 4. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry

    Rat brain sections (1:200), (Cui, Y. et al. (2018) Nature 554, 323.).

    Live cell imaging / Immunocytochemistry
    Expression of Kir4.1 in human U-87 MG cells
    Immunocytochemical staining of live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells using Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
    References
    1. Takumi, T. et al. (1995) J. Biol. Chem. 270, 16339.
    2. Higashi, K. et al. (2001) Am. J. Physiol. 281, C922.
    3. Butt, A.M. and Kalsi, A. (2006) J. Cell Mol. Med. 10, 33.
    Scientific background

    Kir4.1 is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

    The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.

    Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.

    Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1.

    The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. 

    Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney.1,2

    It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the Kreleased during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/11/2018

    Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize Kir4.1 potassium channel from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
    Immunohistochemistry citations
    1. Rat brain sections.
      Cui, Y. et al. (2018) Nature 554, 323.

    Related Products
      • Products for control experiments
        1. Anti-Kir4.1 (KCNJ10) Antibody (#APC-035). This antibody recognizes a different epitope and thus can also be used as a control for detecting Kir4.1 channel.
        2. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#AGP-012). Generated in guinea pig, this antibody the same epitope as #APC-035 and thus can also be used as a control for detecting Kir4.1 channel.
      • Antibodies for immuno-colocalization experiments
        1. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#AGP-012) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #APC-035.
        2. Anti-Kir4.1 (KCNJ10)-ATTO-488 Antibody (#APC-035-AG). A fluorescent labeled primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.