Anti-KCNH2 (erg1) Antibody

KV11.1, Potassium voltage-gated channel subfamily H member 2, Ether-a-go-go-related channel 1, Eag-related protein 1, ERG-1, HERG
    Cat #: APC-016
    Alternative Name KV11.1, Potassium voltage-gated channel subfamily H member 2, Ether-a-go-go-related channel 1, Eag-related protein 1, ERG-1, HERG
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (CY)EEL PAGAP ELPQD GPT, corresponding to residues 1122-1137 of rat KV11.1 (erg1) (Accession O08962). Intracellular, C-terminal part.
    • Anti-KCNH2 (erg1) Antibody
    Accession (Uniprot) Number O08962
    Gene ID 117018
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human, dog, rabbit - 14/16 amino acid residues identical.
    RRID AB_2039911.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-KCNH2 (erg1) Antibody
      Western blot analysis of HEK 293 cell lysate, stably expressing HERG channels:
      1. Anti-KCNH2 (erg1) Antibody (1:200).
      2. Anti-KCNH2 (erg1) Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    • Anti-KCNH2 (erg1) Antibody
      Immunoprecipitation of the lysate of HEK 293 cells, stably expressing HERG with Anti-KCNH2 (erg1) Antibody (#APC-016):
      1. Cell lysate.
      2. Cell lysate + protein A beads + Anti-KCNH2 (erg1) Antibody.
      3. Cell lysate + protein A beads + pre-immune rabbit serum.

      Red arrow indicates the KV11.1 protein while the black arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-KCNH2 (erg1) Antibody.
    Immunohistochemistry
    • Mouse heart sections.
    Immunocytochemistry
    • Mouse cajal cells (Zhu, Y. et al. (2003) Am. J. Physiol285, G1249.).
    References
    1. Curran, M.E. et al. (1995) Cell 80, 795.
    2. Sanguinetti, M.C. et al. (1995) Cell 81, 299.
    3. Pardo, L.A. et al. (2004) Physiology 19, 285.
    4. Gurrola, G.B. et al. (1999) FASEB. J. 13, 953.
    5. Korolkova, Y.V. et al. (2001) J. Biol. Chem. 276, 9868.
    6. Zhou, Z. et al. (1998) Biophys. J. 74, 230.
    Scientific background

    The KV11.1 (HERG) channel is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels that includes the related proteins KV11.2 and KV11.3 (erg2 and erg3). KV11.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains and intracellular N- and C-termini.

    The KV11.1 current is characterized by strong inward rectification with slow activation and very rapid inactivation kinetics. The channel is expressed in the brain and heart (where it underlies the IKr current) and has a central role in mediating repolarization of action potentials.1,2

    Mutations in the KV11.1 channel cause inherited long QT syndrome (LQTS) or abnormalities in the repolarization of the heart that are associated with life-threatening arrhythmias and sudden death. All the identified KV11.1 mutations produce loss of function of the channel via several cellular mechanisms ranging from alterations of gating properties, alterations of channel permeability/selectivity and alterations in intracellular channel trafficking that decreases the number of channels that reach the cell membrane.1,2 

    Recently, drug-induced forms of LQTS have been reported for a wide range of non-cardiac drugs including antihistamines, psychoactive agents and antimicrobials. All these drugs potently block the KV11.1 channel as an unintended side effect, prompting regulatory drug agencies to issue recommendations for the testing of new drugs for their potential KV11.1 blocking effect.

    In addition, KV11.1 expression was found to be upregulated in several tumor cell lines of different histogenesis, suggesting that it confers the cells some advantage in cell proliferation. Indeed, in several studies it has been shown that inhibition of the KV11.1 current leads to a decrease in tumor cell proliferation.3

    Several toxins from scorpion venoms are potent blockers (affecting the channels in the nanomolar range) of KV11.1 channels. Among these the most potent and selective are Ergtoxin-1 (16 nM)4 and BeKM-1 (3 nM).5 In addition, the methanesulfonanilide class III antiarrhythmic agent E-4031 also blocks KV11.1 channel in the nanomolar range (7.7 nM).6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-KCNH2 (erg1) Antibody (#APC-016) is a highly specific antibody directed against an epitope of the rat KV11.1 channel. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize KV11.1 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. HEK-293 transfected cells.
      Zhang, Y. et al. (2016) Biochem. Pharmacol. 113, 24.
    More product citations
    1. White, E.J. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 295, G691.
    2. Melnyk, P. et al. (2005) Cardiovasc. Res. 65, 104.
    3. Bellocq, C. et al. (2004) Mol. Pharmacol. 66, 1093.
    4. Wang, J. et al. (2004) J. Biol. Chem. 279, 13289.
    5. Crociani, O. et al. (2003) J. Biol. Chem. 278, 2947.
    6. Ficker, E. et al. (2003) Circ. Res. 92, 87.
    7. Milnes, J.T. et al. (2003) Br. J. Pharmacol. 139, 887.
    8. Papa, M. et al. (2003) J. Comp. Neurol. 466, 119.
    9. Wang, J. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284, 256.
    10. Zhu, Y. et al. (2003) Am. J. Physiol. 285, G1249.
    11. Cayabyab, F.S. et al. (2002) J. Biol. Chem. 277, 13673.
    12. Pond, A.L. et al. (2000) J. Biol. Chem. 275, 5997.
    13. Akbarali, H.I. et al. (1999) Am. J. Physiol. 277, C1284.
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