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Anti-mGluR7 (extracellular) Antibody

Metabotropic glutamate receptor 7, GRM7, mGlu7, Gprc1g

Cat #: AGC-017
Alternative Name Metabotropic glutamate receptor 7, GRM7, mGlu7, Gprc1g
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide TISGSKKEDTDRKC, corresponding to amino acid residues 377-390 of rat mGluR7 (Accession P35400). Extracellular, N-terminus.
Accession (Uniprot) Number P35400
Gene ID 81672
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_11124483.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 4) and rat (lanes 2 and 5) brain membranes and human CCF-STTGI astrocytoma (lanes 3 and 6) cell line lysate (1:200):
    Western blot analysis of mouse (lanes 1 and 4) and rat (lanes 2 and 5) brain membranes and human CCF-STTGI astrocytoma (lanes 3 and 6) cell line lysate (1:200):
    1-3. Anti-mGluR7 (extracellular) Antibody (#AGC-017), (1:200).
    4-6. Anti-mGluR7 (extracellular) Antibody, preincubated with mGluR7 (extracellular) Blocking Peptide (#BLP-GC017).
Immunohistochemistry
  • Expression of mGluR7 in rat neocortex
    Expression of mGluR7 in rat neocortex
    Immunohistochemical staining of rat neocortex frozen sections using Anti-mGluR7 (extracellular) Antibody (#AGC-017). A. mGluR7 staining (green) appears in several neocortical neurons. B. Merge image showing mGluR7 staining together with cell nuclei (blue).
Indirect flow cytometry
  • Cell surface detection of mGluR7 in live intact human T cell leukemia (Jurkat) cell line:
    Cell surface detection of mGluR7 in live intact human T cell leukemia (Jurkat) cell line:
    ___ Unstained cells + goat-anti-rabbit-PE.
    ___ Cells + Anti-mGluR7 (extracellular) Antibody (#AGC-017), (1:20) + goat-anti-rabbit-PE.
  • The blocking peptide in not suitable for this application.
Live cell imaging / Immunocytochemistry
  • Expression of mGluR7 in rat PC12 cells
    Expression of mGluR7 in rat PC12 cells
    Cell surface detection of mGluR7 in live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-mGluR7 (extracellular) Antibody (#AGC-017), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of the two pictures.
References
  1. Kim, C.H. et al. (2008) J. Neurosci. Res. 86, 1.
  2. Niswender, C.M. and Conn, P.J. (2010) Annu. Rev. Pharmacol. Toxicol. 50, 295.
  3. Nicoletti, F. et al. (2011) Neuropharmacology 60, 1017.
  4. O’Connor, R.M. et al. (2010) Eur. J. Pharmacol. 639, 123.
Scientific background

Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1.

The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2. Indeed, mGluR7 can be detected as five different splice variants3.

Like all group III metabotropic glutamate receptors, mGluR7 is localized presynaptically. It is highly expressed in the central nervous system and could also be detected in some peripheral organs like hair cells and spiral ganglion cells of the inner ear3.

As opposed to other group III metabotropic glutamate receptors, mGluR7 has very low affinity for its substrate L-glutamate, therefore, only under high synaptic activity is the receptor active. This enables the receptor to act as a negative feedback loop by inhibiting the release of L-glutamate3,4.
mGluR7 may play a molecular role in the pathways leading to emotion and cognition4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR7. Anti-mGluR7 (extracellular) Antibody (#AGC-017) can be used in western blot, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize mGluR7 from human, rat and mouse samples.

For research purposes only, not for human use
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