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Anti-mGluR8 (extracellular) Antibody

Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H

Cat #: AGC-028
Alternative Name Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8 (Accession P70579). Extracellular, N-terminus.
Accession (Uniprot) Number P70579
Gene ID 60590
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, human – 12/13 amino acid residues identical.
RRID AB_2340949.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
    Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
    1-3. Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:400).
    4-6. Anti-mGluR8 (extracellular) Antibody, preincubated with mGluR8 (extracellular) Blocking Peptide (#BLP-GC028).
Immunohistochemistry
  • Expression of mGluR8 in rat globus pallidus
    Expression of mGluR8 in rat globus pallidus
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:100). A. Staining reveals expression of mGluR8 (red) in globus pallidus neurons (arrow) and their dendrites. B. The same section was also stained with DAPI (blue) revealing cell nuclei. C. Merge of the two images.
References
  1. Conn, P.J. et al. (1997) Annu. Rev. Pharmacol. Toxicol. 37, 205.
  2. Somogyi, J. et al. (2004) Eur. J. Neurosci. 19, 552.
  3. Saugstad, J.A. et al. (1997) Mol. Pharmacol. 51, 119.
  4. Zhai, J. et al. (2002) Neuropharmacology 43, 223.
Scientific background

Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission1. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gαi/o to inhibit adenylyl cyclase found in the presynaptic active zone2.

The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina3. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus4. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels.

Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR8. Anti-mGluR8 (extracellular) Antibody (#AGC-028) can be used in western blot and immunohistochemistry applications. It has been designed to recognize mGluR8 from rat, mouse and human samples.

For research purposes only, not for human use
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