Anti-Neuropilin-1 (extracellular) Antibody

Vascular Endothelial Cell Growth Factor 165 Receptor, VEGF165R, CD304, NRP1
    Cat #: ANR-063
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)GGKHRENKVFMRK, corresponding to amino acid residues 502 - 514 of human Neuropilin-1 (Accession O14786). Extracellular, N-terminus.
    Accession (Uniprot) Number O14786
    Gene ID 8829
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate  (lanes 2 and 4):
    1, 2. Anti-Neuropilin-1 (extracellular) Antibody (#ANR-063), (1:200).
    3, 4. Anti-Neuropilin-1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human Jurkat T-cell leukemia cell line lysate:
    1. Anti-Neuropilin-1 (extracellular) Antibody (#ANR-063), (1:200).
    2. Anti-Neuropilin-1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of Neuropilin-1 in human U-87 MG cells
    Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-Neuropilin-1 (extracellular) Antibody (#ANR-063), (1:25). B. Live view of the cells.
    References
    1. Pellet-Many, C. et al. (2008) Biochem. J. 411, 211.
    Scientific background

    Neuropilins (NRPs) are co-receptors for class 3 semaphorins, polypeptides with key roles in axonal guidance, and for members of the VEGF (vascular endothelial growth factor) family of angiogenic cytokines.

    Neuropilin 1 (NRP1) is a transmembrane glycoprotein and comprises of a large extracellular region containing multiple domains, a single transmembrane domain and small cytoplasmic domain, also with a number of protein interacting domains.

    Class 3 semaphorins bind NRP1 (and NRP2) with different, and partially overlapping, specificities and affinities. Plexin A1 and Plexin A2 are the major signaling receptors for class 3 semaphorins, and complex with NRPs and semaphorins to form a holoreceptor essential for mediating effects of semaphorins on growth-cone collapse and axonal guidance in neurogenesis. The major VEGF-A signaling receptor, VEGF receptor 2, forms complexes with NRP1 and is important for optimal VEGFR2 signaling and function in endothelial cells.

    Targeted disruption of the NRP1 gene has demonstrated an essential dual role of these molecules in neurogenesis and cardiovascular development. NRP1-null mice die with a spectrum of cardiovascular and neuronal defects1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 29/10/2018

    Anti-Neuropilin-1 (extracellular) Antibody (#ANR-063) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications. It has been designed to recognize NRP1 from human, mouse, and rat samples.

    For research purposes only, not for human use