Anti-Neuropilin-1 (NRP1) (extracellular) Antibody

Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
    Cat #: ANR-063
    Alternative Name Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)GGKHRENKVFMRK, corresponding to amino acid residues 502-514 of human neuropilin-1 (Accession O14786). Extracellular, N-terminus.
    • Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
    Accession (Uniprot) Number O14786
    Gene ID 8829
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    RRID AB_2756695.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, lci, wb
    May also work in: ifc*, ih*, ip*
    Western blot
    • Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
      1,2. Anti-Neuropilin-1 (NRP1) (extracellular) Antibody (#ANR-063), (1:200).
      3,4. Anti-Neuropilin-1 (NRP1) (extracellular) Antibody preincubated with the negative control antigen.
    • Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
      Western blot analysis of human Jurkat T-cell leukemia cell line lysate:
      1. Anti-Neuropilin-1 (NRP1) (extracellular) Antibody (#ANR-063), (1:200).
      2. Anti-Neuropilin-1 (NRP1) (extracellular) Antibody, preincubated with the negative control antigen.
    Live cell imaging / Immunocytochemistry
    • Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
      Expression of Neuropilin-1 in human U-87 MG cells
      Cell surface detection of Neuropilin-1 in live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-Neuropilin-1 (NRP1) (extracellular) Antibody (#ANR-063), (1:25). B. Live view of the cells.
    1. Pellet-Many, C. et al. (2008) Biochem. J. 411, 211.
    Scientific background

    Neuropilins (NRPs) are co-receptors for class 3 semaphorins, polypeptides with key roles in axonal guidance, and for members of the VEGF (vascular endothelial growth factor) family of angiogenic cytokines.

    Neuropilin 1 (NRP1) is a transmembrane glycoprotein and comprises a large extracellular region containing multiple domains, a single transmembrane domain and a small cytoplasmic domain, also with a number of protein interacting domains.

    Class 3 semaphorins bind NRP1 (and NRP2) with different, and partially overlapping, specificities and affinities. Plexin A1 and Plexin A2 are the major signaling receptors for class 3 semaphorins, and complex with NRPs and semaphorins to form a holoreceptor essential for mediating effects of semaphorins on growth-cone collapse and axonal guidance in neurogenesis. The major VEGF-A signaling receptor, VEGF receptor 2, forms complexes with NRP1 and is important for optimal VEGFR2 signaling and function in endothelial cells.

    Targeted disruption of the NRP1 gene has demonstrated an essential dual role of these molecules in neurogenesis and cardiovascular development. NRP1-null mice die with a spectrum of cardiovascular and neuronal defects1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Neuropilin-1 (NRP1) (extracellular) Antibody (#ANR-063) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications. It has been designed to recognize NRP1 from human, mouse, and rat samples.

    For research purposes only, not for human use
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