Anti-Plexin-A2 (extracellular) Antibody

PLXNA2, PLXN2, Semaphorin receptor OCT
    Cat #: APR-082
    Alternative Name PLXNA2, PLXN2, Semaphorin receptor OCT
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)RDPESSAMLDYELH, corresponding to amino acid residues 205-218 of mouse PLXNA2 (Accession P70207). Extracellular, N-terminus.
    • Anti-Plexin-A2 (extracellular) Antibody
    Accession (Uniprot) Number P70207
    Gene ID 18845
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    RRID AB_2756767.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-Plexin-A2 (extracellular) Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
      1,2. Anti-Plexin-A2 (extracellular) Antibody (#APR-082), (1:200).
      3,4. Anti-Plexin-A2 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Plexin-A2 (extracellular) Antibody
      Immuno-colocalization of Plexin-A2 and p75NTR in rat brain
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Plexin-A2 (extracellular) Antibody (#APR-082), (1:400) and mouse Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170), (1:300). A. Sections of rat horizontal diagonal band (HDB) were stained for PLXNA2 (green). B. Same sections were stained for p75NTR (red). C. Merge of the two images reveals cells expressing both PLXNA2 and p75NTR (horizontal arrows), cells expressing only PLXNA2 (upwards pointing arrows) or cells expressing only p75NTR (downwards pointing arrows) can also be observed. Cell nuclei are stained with DAPI (blue).
    1. Tamagnone, L. et al. (1999) Cell 99, 71.
    2. Janssen, B.J. et al. (2012) Nat. Struct. Mol. Biol. 19, 1293.
    3. Nogi, T. et al. (2010) Nature 467, 1123.
    4. Tian, T.V. et al. (2014) Oncogene 33, 2204.
    Scientific background

    The Plexin gene family is comprised of at least 9 members in 4 subfamilies. Plexins are widely expressed in neurons but have been also detected in epithelial cells1. Plexins closely interact with a large family of proteins called Semaphorins which control a variety of processes in the CNS including cell migration and axonal growth.

    Plexins and Semaphorins share a common feature of a conserved 500 amino acid “sema” domain2. The ectodomain of PLXNA2 is an N-terminal seven bladed β-propeller sema domain followed by a cysteine rich PSI (plexin-semaphorin-integrin) domain. PLXNA2 has four N-terminal domains and unlike semaphorins the sema domain of unliganded PLXNA2 does not homodimerize readily.

    Many of the functions of Class 3 semaphorins (Sema3A-Sema3G) are mediated by Plexin-A2 (PLXNA) and other A-plexins. Sema3 signaling depends on semaphorin interacting with one of the two Nrp co-receptors that associate with PLXNA to generate holoreceptors.

    PLXNA also interacts with semaphorin 6A (Sema6A). Before binding, the Sema6A ectodomain can be found in a ‘face-to-face’ homodimer arrangement, similar to the arrangement in Sema3, whereas PLXNA is in an unexpected ‘head-on’ homodimer arrangement. The structure of the Sema6A–PLXNA signaling complex is a 2:2 heterotetramer3.

    In prostate cancer TMPRSS:ERG gene fusion causes aberrant expression of the transcription factor ERG and the induction of cell migration and invasion. TMPRSS:ERG upregulates PLXNA expression and elevated PLXNA levels were found in prostate cancer cells. Levels of PLXNA were even higher in metastatic prostate cancer than in localized tumors suggesting PLXNA may play a role in the disease’s development.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Plexin-A2. Anti-Plexin-A2 (extracellular) Antibody (#APR-082) can be used in western blot and immunohistrochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting PLXNA2 in living cells. It has been designed to recognize PLXNA2 from rat, mouse and human samples.

    For research purposes only, not for human use
    Shipping and Ordering information