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Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody

Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor

Cat #: APR-064
Alternative Name Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor (Accession Q62928). 3rd extracellular loop.
Accession (Uniprot) Number Q62928
Gene ID 81752
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
RRID AB_2341058.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), rat stomach (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), rat stomach (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:
    1-3. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:500).
    4-6. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody, preincubated with Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064).
  • Western blot analysis of human THP-1 acute monocytic leukemia cell lysates (lanes 1 and 4), human Jurkat T cell leukemia cell lysates (lanes 2 and 5) and human HT-29 colorectal adenocarcinoma cell lysates (lanes 3 and 6):
    Western blot analysis of human THP-1 acute monocytic leukemia cell lysates (lanes 1 and 4), human Jurkat T cell leukemia cell lysates (lanes 2 and 5) and human HT-29 colorectal adenocarcinoma cell lysates (lanes 3 and 6):
    1-3. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:500).
    4-6. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody, preincubated with Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064).
Immunohistochemistry
  • Expression of Prostanoid EP2 receptor in rat cerebellum
    Expression of Prostanoid EP2 receptor in rat cerebellum
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:100). Prostanoid EP2 receptor staining (red) is detected in Purkinje neurons and their dendritic tree (arrow). Blue is DAPI nissl counterstain.
Indirect flow cytometry
  • Cell surface detection of Prostanoid EP2 receptor in live intact human Jurkat T cell leukemia cell line:
    Cell surface detection of Prostanoid EP2 receptor in live intact human Jurkat T cell leukemia cell line:
    ___ Unstained cells + goat anti-rabbit-AlexaFluor-488 secondary antibody.
    ___ Cells + Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:15) + goat anti-rabbit-AlexaFluor-488 secondary antibody.
  • The blocking peptide is not suitable for this application.
References
  1. Campbell, W.B. et al. (1990) The Pharmacological Basis of Therapeutic 8, 600.
  2. Davies, P. et al. (1992) Inflammation: Basic Principles and Clinical Correlates 2, 123.
  3. Coleman, R.A. et al. (1992) Comprehensive Medicinal Chemistry 3, 123.
  4. Ferreri, N.R. et al. (1992) J. Biol. Chem. 267, 9443.
  5. Bastien, L. et al. (1994) J. Biol. Chem. 269, 11673.
Scientific background

Prostaglandin E2 (PGE2) is involved in a number of physiological and pathophysiological events in many tissues throughout the body1. The physiological actions of PGE2 are mediated through its interaction with cell surface prostaglandin E receptors.

There are three pharmacologically defined subtypes of the EP receptor, EP1, EP2, and EP3, and these subtypes are suggested to be different in their signal transduction2. These receptors belong to the G-protein coupled receptor (GPCR) superfamily. Like all members they have seven transmembrane domains with an extracellular N-terminal tail and an intracellular C-terminus. The EP2 receptor is expressed in the vasculature, the gastrointestinal tract, kidney and also in the ciliary muscles of the eye3.

PGE2 is known to play a central role in the pathophysiology of inflammation in synergy with other proinflammatory mediators. PGE2 inhibits the function and the proliferation of T cells and the histamine release from mast cells by increasing the intracellular level of cAMP4.

The EP2 subtype is thought to be in part responsible for vasodilation, oedema formation, hyperanalgesia, modulation of the immune system, and the breakdown of bone and cartilage associated with disorders such as rheumatoid arthritis5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize EP2 receptor from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Mouse spinal cord sections.
    Cantaut-Belarif, Y. et al. (2017) J. Cell Biol. 216, 2979.
Indirect flow cytometry citations
  1. Human Blood eosinophils.
    Durchschein, F. et al. (2019) Dig. Dis. Sci. 64, 2806.
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