Anti-Prostanoid EP2 Receptor (extracellular) Antibody

Prostaglandin E2 Receptor EP2 subtype, PTGER2
    Cat #: APR-064
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor (Accession Q62928). 3rd extracellular loop.
    Accession (Uniprot) Number Q62928
    Gene ID 81752
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, ih, wb
    May also work in: ic, ip, lci
    Western blot
    Western blot analysis of rat brain lysate (lanes 1 and 4), rat stomach  lysate (lanes 2 and 5) and mouse kidney lysate (lanes 3 and 6):
    1-3. Anti-Prostanoid EP2 Receptor (extracellular) Antibody (#APR-064), (1:500).
    4-6. Anti-Prostanoid EP2 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human THP-1 acute monocytic leukemia cell lysates (lanes 1 and 4), human Jurkat T cell leukemia cell lysates (lanes 2 and 5) and human HT-29 colorectal adenocarcinoma cell lysates (lanes 3 and 6):
    1-3. Anti-Prostanoid EP2 Receptor (extracellular) Antibody (#APR-064), (1:500).
    4-6. Anti-Prostanoid EP2 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Prostanoid EP2 Receptor in rat cerebellum
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Prostanoid EP2 Receptor (extracellular) Antibody (#APR-064), (1:100). Prostanoid EP2 Receptor staining (red) is detected inPurkinje neurons and their dendritic tree (arrow). Blue is DAPI nissl counterstain.
    Indirect flow cytometry
    Indirect flow cytometry analysis of live intact human Jurkat T cell leukemia cell line:
    ___ Unstained cells + goat anti-rabbit-AlexaFluor-488 secondary antibody.
    ___ Cells + Anti-Prostanoid EP2 Receptor (extracellular) Antibody (#APR-064), (1:15) + goat anti-rabbit-AlexaFluor-488 secondary antibody.
    The control antigen is not suitable for this application.
    References
    1. Campbell, W.B. et al. (1990) The Pharmacological Basis of Therapeutic 8, 600.
    2. Davies, P. et al. (1992) Inflammation: Basic Principles and Clinical Correlates 2, 123.
    3. Coleman, R.A. et al. (1992) Comprehensive Medicinal Chemistry 3, 123.
    4. Ferreri, N.R. et al. (1992) J. Biol. Chem. 267, 9443.
    5. Bastien, L. et al. (1994) J. Biol. Chem. 269, 11673.
    Scientific background

    Prostaglandin E2 (PGE2) is involved in a number of physiological and pathophysiological events in many tissues throughout the body1. The physiological actions of PGE2 are mediated through its interaction with cell surface prostaglandin E receptors.

    There are three pharmacologically defined subtypes of the EP receptor, EP1, EP2, and EP3, and these subtypes are suggested to be different in their signal transduction2. These receptors belong to the G-protein coupled receptor (GPCR) superfamily. Like all members they have seven transmembrane domains with extracellular N-terminal tail and intracellular C-terminus. The EP2 receptor is expressed in the vasculature, the gastrointestinal tract, kidney and also in the ciliary muscles in the eye3.

    PGE2 is known to play a central role in the pathophysiology of inflammation in synergy with other proinflammatory mediators. PGE2 inhibits the function and the proliferation of T cells and the histamine release from mast cells by increasing the intracellular level of cAMP4. EP2 subtype is thought to be in part responsible for vasodilation, oedema formation, hyperanalgesia, modulation of the immune system, and the breakdown of bone and cartilage associated with disorders such as rheumatoid arthritis5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope rat Prostaglandin EP2 Receptor subtype. Anti-Prostanoid EP2 Receptor (extracellular) Antibody (#APR-064) can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize Prostanoid E2 Receptor from rat, mouse and human samples.

    For research purposes only, not for human use