Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody

p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271
Cat #: AN-170-F
Alternative Name p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271
  • Lyophilized Powder
  • Type: Monoclonal
    Source: Mouse
    Reactivity: r
      • Solubilized proteins from PC12 membranes.
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Purity Purified from cultured hybridoma medium on immobilized rProtein G.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype IgG1.
    Specificity 192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin.
    Label Fluorescein isothiocyanate (FITC).
    Clone 192-IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AN-170), and direct flow cytometry (labeled antibody).
    Applications: fc
      • Cell surface detection of p75NTR in live PC12 cells:
        ___ Unstained cells.
        ___ Cells + Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody (#AN-170-F) 1 µl/1x106 cells (1:50).
      • p75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for β-amyloid, and for the myelin axon repellant protein MAG.1,2,4,6 

        Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. 

        p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1,3,6 

        Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain.

        p75NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity, and is more potently activated by pro-neurotrophins.5  

        Overexpression of the intracellular domain in developing neurons induces apoptosis.  

        NGF-dependent association of NRIF with p75NTR contributes to pro-apoptotic signaling.7,8 β-amyloid peptide binds p75NTR and stimulates pro-apoptotic signaling by p75NTR, suggesting that the receptor may be relevant for Alzheimer's disease.9

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 14/07/2019

    Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170) is a highly specific monoclonal antibody directed against p75NTR. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize p75NTR from rat samples only.

    Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody (#AN-170-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody was specifically developed to be used in direct flow cytometry applications, especially for those that use multiple and simultaneous labeling of different markers.

    For research purposes only, not for human use
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