- Peptide (C)RHKHLAKDHSPDLYS, corresponding to amino acid residues 200 - 214 of mouse Renin Receptor (Accession Q9CYN9). Extracellular, N-terminus.
- Rat heart and brain membranes; mouse kidney membranes and BV-2 microglia cell lysate; human THP-1 monocytic leukemia cell lysate (1:200-1:1000).
- Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):1-3. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200).
4-6. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
- Human THP-1 monocytic leukemia cells (2.5 µg).
The (pro) Renin receptor, (p)RR, encoded by ATP6AP2 gene in humans, is a single pass transmembrane protein that binds both Renin and its inactive precursor pro-renin. ATP6AP2 is highly conserved among species, and (Pro) Renin Receptor orthologues are expressed in species from mammals to C. elegans and Drospohila melanogaster1.
Binding of Pro-renin to (p)RR receptor facilitates its truncation and generation of Renin. Renin is an aspartyl protease that cleaves angiotensinogen into angiotensin I, the rate-limiting reaction in the cascade generating angiotensin, a peptide hormone that is involved in blood pressure regulation. Renin binding by (P)RR affects important biological systems and pathways that are involved in cell cycle regulation, autophagy, acid-base balance, energy metabolism, embryonic development, T cell homeostasis, water balance, blood pressure regulation, cardiac remodeling and maintenance of podocyte structure1.
In addition, (P)RR has been reported to contribute to the pathogenesis of diseases such as fibrosis, hypertension, pre-eclampsia, diabetic microangiopathy, acute kidney injury, cardiovascular disease, cancer and obesity1.
The receptor is widely distributed in organs throughout the body, including the kidneys, heart, brain, eyes, placenta, and the immune system1.