Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody

AT1 Receptor, AT1R, AGTR1
    Cat #: AAR-011-F
    Alternative Name AT1 Receptor, AT1R, AGTR1
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
    • Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
    Accession (Uniprot) Number P30556
    Gene ID 185
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat, rabbit, dog - identical.
    RRID AB_2756538.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-011) and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    • Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
      Cell surface detection of AT1 receptor in live intact human THP-1 monocytic leukemia cells:
      ___ Cells alone.
      ___ Cells + Rabbit IgG isotype control-FITC.
      ___ Cells + Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody (#AAR-011-F), (5 µg).
    • Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody
      Cell surface detection of AT1 receptor in live intact human Jurkat T-cell leukemia cells:
      ___ Cells alone.
      ___ Cells + Rabbit IgG isotype control-FITC.
      ___ Cells + Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody (#AAR-011-F), (5 µg).
    1. de Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.
    2. Balakumar, P. and Jagadeesh, G. (2014) J. Mol. Endocrinol. 53, R71.
    3. Graus-Nunes, F. et al. (2017) Mol. Cell. Endocrinol. 439, 54.
    Scientific background

    The biological functions of angiotensin II are mediated by AT1 and AT2 receptors. Both belong to the G-protein coupled receptor superfamily and are comprised of seven hydrophobic transmembrane segments forming α helices in the lipid bilayer of the cell membrane, an extracellular N-terminus and an intracellular N-terminus1.

    Two highly homologous isoforms of the receptor, AT1A and AT1B have been identified in rodents. The AT1A receptor accounts for 90% of the total binding, and is predominant in the kidney, vascular smooth muscle cells, heart, liver, and in some areas of the brain, while the AT1B receptor is found predominantly in the pituitary and adrenal glands, placenta, lung, and brain. These isoforms are pharmacologically indistinguishable and are both selectively antagonized by losartan.

    Through AT1 receptor, angiotensin II stimulates multiple signaling pathways, cross-talks with several tyrosine kinases, and trans-activates growth factor receptors. Phosphorylation of the receptor by GPCR kinases terminates receptor activation and promotes β-arrestin recruitment. β-arrestin-scaffolded signaling mediates ‘secondary signaling’ that involves multiple kinases that link to cell protective downstream signaling molecules2.

    In a model of diet-induced obese mice, AT1 receptor inhibition demonstrates beneficial pleiotropic effects. Mice receiving the treatment exhibit enhanced pancreatic duodenal homeobox 1 (PDX1) and GLP-1 pancreatic islet expression. PDX1 islets are essential for the expression of glucokinase and GLUT2. Greater GLP-1 levels, islet vascularization and reduced apoptosis and macrophage infiltration are also observed3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize AT1 receptor from rat, mouse, and human samples.

    Anti-Angiotensin II Receptor Type-1 (extracellular)-FITC Antibody (#AAR-011-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

    For research purposes only, not for human use
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