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Anti-Semaphorin 6A (extracellular) Antibody

SEMAQ, SEMA6A-1, SEMA6A

Cat #: ASR-071
Alternative Name SEMAQ, SEMA6A-1, SEMA6A
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)NHQDKKGVIRESYLK, corresponding to amino acid residues 626 - 640 of mouse Semaphorin 6A (Accession O35464). Extracellular, N-term.
Accession (Uniprot) Number O35464
Gene ID 20358
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human  – identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071), (1:200).
    3-4. Anti-Semaphorin 6A (extracellular) Antibody, preincubated with Semaphorin 6A (extracellular) Blocking Peptide (BLP-SR071).
  • Western blot analysis of human Malme-3M melanoma cell line lysate (lanes 1 and 3) and human Colo-205 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):
    Western blot analysis of human Malme-3M melanoma cell line lysate (lanes 1 and 3) and human Colo-205 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):
    1-2. Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071), (1:200).
    3-4. Anti-Semaphorin 6A (extracellular) Antibody, preincubated with Semaphorin 6A (extracellular) Blocking Peptide (BLP-SR071).
Immunohistochemistry
  • Expression of Semaphorin 6A in rat cerebellum.
    Expression of Semaphorin 6A in rat cerebellum.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071), (1:400), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Semaphorin 6A immunoreactivity (red) appears in the molecular layer (MOL) and in the Purkinje cell layer (P) (arrows).  B. Pre-incubation of the antibody with Semaphorin 6A (extracellular) Blocking Peptide (BLP-SR071), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer, P = Purkinje cell layer
  • Expression of Semaphorin 6A in rat spinal cord.
    Expression of Semaphorin 6A in rat spinal cord.
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071), (1:400), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Staining in the dorsal horn region, showed Semaphorin 6A immunoreactivity (red) in layers 1-2 (L1-2 and arrows).  B. Pre-incubation of the antibody with Semaphorin 6A (extracellular) Blocking Peptide (BLP-SR071), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Togari, A., et al., (2000) Brain Res, 878(1-2): p. 204-9.
  2. Sun, Q., et al., (2018) J Am Heart Assoc,  7(11).
  3. Shima, D.T. and C. Mailhos, (2000) Curr Opin Genet Dev, 10(5): p. 536-42.
  4. Xiang, R.H., et al., (1996) Genomics, 32(1): p. 39-48.
  5. Hall, K.T., et al., (1996) Proc Natl Acad Sci U S A, 93(21): p. 11780-5.
  6. Kerjan, G., et al., (2005) Nat Neurosci, 8(11): p. 1516-24.
  7. Leighton, P.A., et al., (2001) Nature, 410(6825): p. 174-9.
  8. Little, G.E., et al., (2009) PLoS Biol, 7(4): p. e98.
  9. Perez-Branguli, F., et al., (2016) PLoS One, 11(7): p. e0158686.
  10. Runker, A.E., et al., (2008) Neural Dev, 3: p. 34.
  11. Runker, A.E., et al., (2011) PLoS One, 6(11): p. e26488.
  12. Suto, F., et al., (2007) Neuron, 53(4): p. 535-47.
  13. Matthes, D.J., et al., (1995) Cell, 81(4): p. 631-9.
  14. Tamagnone, L., et al., (1999) Cell, 99(1): p. 71-80.
  15. Kolodkin, A.L., (1993) Cell, 75(7): p. 1389-99.
  16. de Castro, F., et al. (1999) J Neurosci, 19(11): p. 4428-36.
  17. Kolodkin, A.L., et al., (1992) Neuron, 9(5): p. 831-45.
  18. Luo, Y., D. Raible, and J.A. Raper, (1993) Cell, 75(2): p. 217-27.
  19. Messersmith, E.K., et al., (1995) Neuron, 14(5): p. 949-59.
  20. Gilabert-Juan, J., et al., (2015) Psychiatry Res, 229(3): p. 850-7.
Scientific background

Semaphorin 6A (Sema6A) is a member of the semaphorin family of genes involved in cell migration, axon guidance, development of bone1,2, vasculature3, cancer metastasis4, B-cell aggregation and differentiation5, and synaptogenesis6-12, as well as in inhibiting synaptic terminal arborization13.

The semaphorins represent one of the largest families of axon guidance cues consisting of eight classes. Members are classified according to structural criteria. All semaphorins share a highly conserved Sema-domain of about 500 amino acids14 with 17 conserved cysteines. Classes 1 and 2 are found only in invertebrates, while classes 3 to 7 contain the vertebrate family members, and class V members are encoded by viral genomes. Semaphorins include both transmembrane (classes 1, 4, 5 and 6), glycosylphosphatidyl inositol (class 7) and secreted (classes 2, 3 and V) proteins15.

Initially, all semaphorin family members were believed to induce inhibitory actions on axon pathfinding, branching or targeting, but there is increasing evidence that semaphorins may also have a role in chemoattraction13,16-19. Mutations of Sema6A in mice is associated with a spectrum of subtle defects in cell migration and axon guidance in various brain areas, including the thalamocortical system, hippocampus, cerebellum and various other structures6-12,20. Previous study of gene expression analysis revealed alterations in semaphorin and plexin expression in the prefrontal cortex of patients with schizophrenia20.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 22/05/2022

Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Semaphorin 6A from rat, mouse and human samples.

For research purposes only, not for human use
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