Anti-TRPC3-ATTO-594 Antibody

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3
    Cat #: ACC-016-AR
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1). Intracellular, C-terminus.
    Accession (Uniprot) Number Q9QZC1
    Gene ID 22065
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, rat - 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-016), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic
    Immunohistochemistry
    Expression of TRPC3 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR), (1:50). Staining is present in neuronal cell bodies. Hoechst 33342 is used as the counterstain.
    Colocalization of TRPC6 and TRPC3 in rat cerebellum
    Immunohistochemical staining of rat cerebellum frozen section using Guinea pig Anti-TRPC6 Antibody (#AGP-002) and rabbit Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR). A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section as in A, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates co-localization in Purkinje cells and molecular layer.
    Immuno-colocalization of VMAT2 and TRPC3 in rat brain
    Immunohistochemical staining of perfusion-fixed frozen rat substantia nigra sections using Anti-VMAT2-ATTO-488 Antibody (#AMT-006-AG), (1:60) and Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR), (1:60). A. VMAT2 staining (green). B. The same section stained for TRPC3 (red). C. Merged image demonstrates the ubiquitous colocalization of VMAT2 and TRPC3 in cells of the substantia nigra pars compacta. Arrows point at examples of co-expression.
    References
    1. Moran, M.M. et al. (2004) Current Opin. Neurobiol14, 362.
    2. Clapham, D.E. et al. (2003) Pharmacol. Rev55, 591.
    3. Clapham, D.E. (2003) Nature 426, 517.
    4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci117, 5707.
    5. Huang, C.L. (2004) J. Am. Soc. Nephrol15, 1690.
    6. Vazquez, G. et al. (2003) J. Biol.Chem278, 21649.
    7. Venkatachalam, K. et al. (2003) J. Biol.Chem278, 29031.
    Scientific background

    The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel family and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4

    The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: TRPC1; TRPC4 and TRPC5; TRPC3, TRPC6, TRPC7 and TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

    TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.

    TRPC3 was shown to be activated by DAG and SOC, in heterologous expression systems.6,7

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-TRPC3-ATTO-594 Antibody
    Immuno-colocalization of mGluR1 and TRPC3 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse cerebellum sections using Anti-TRPC3-ATTO-594 Antibody (ACC-016-AR), (1:60) and Anti-mGluR1 (extracellular)-ATTO-488 Antibody (#AGC-006-AG), (1:60).  A. TRPC3 staining (red). B. mGluR1 staining (green). C. Merge of the two images suggests extensive co-localization in Purkinje cells (vertical arrows). Note expression of mGluR1 in Purkinje dendrites (horizontal arrow) but not of TRPC3. Cell nuclei are stained with DAPI (blue).

    Last update: 22/11/2018

    Anti-TRPC3 Antibody (#ACC-016) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize TRPC3 from mouse, rat, and human samples.

    Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPC3-ATTO-594 Antibody can be used in immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use