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The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel family and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4
The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: TRPC1; TRPC4 and TRPC5; TRPC3, TRPC6, TRPC7 and TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.
TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.
TRPC3 was shown to be activated by DAG and SOC, in heterologous expression systems.6,7
Species reactivity key:
Immuno-colocalization of mGluR1 and TRPC3 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse cerebellum sections using Anti-TRPC3-ATTO-594 Antibody (ACC-016-AR), (1:60) and Anti-mGluR1 (extracellular)-ATTO-488 Antibody (#AGC-006-AG), (1:60). A. TRPC3 staining (red). B. mGluR1 staining (green). C. Merge of the two images suggests extensive co-localization in Purkinje cells (vertical arrows). Note expression of mGluR1 in Purkinje dendrites (horizontal arrow) but not of TRPC3. Cell nuclei are stained with DAPI (blue).
Anti-TRPC3 Antibody (#ACC-016) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize TRPC3 from mouse, rat, and human samples.
Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPC3-ATTO-594 Antibody can be used in immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.
Products for control experiments
- Anti-TRPC3 Antibody (#ACC-016), (for western blot analysis).
- Blockers/Antagonists: small molecules
- Explorer kits