Name: Tertiapin-Q-ATTO Fluor-633
Brand: Alomone Labs
SKU: STT-170-FR

Cat #: STT-170-FR

Lyophilized Powder yes

Bioassay Tested

Origin Synthetic peptide

MW: 3159.6 Da

Purity: >95% (HPLC)

Effective concentration 50-200 nM.

Sequence ALCNCNRIIIPHQCWKKCGKK

Modifications Disulfide bonds between Cys³-Cys¹⁴, Cys⁵-Cys¹⁸. Lys²¹- C-terminal amidation.

Label ATTO-633. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO-633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well known dye Alexa 647, Alexa 633 and Cy5. The extent of labeling is one molecule of dye per molecule of Tertiapin-Q.

Structure

Activity Tertiapin-Q blocks a range of inward rectifier K⁺ channels (K_ir), in particular ROMK1 and GIRK, but with no effect on K_ir2 family members^1,2. In addition, it was shown to inhibit acetylcholine induced K⁺ currents in the heart^3,4.

References-Activity

Jin, W. and Lu, Z. (1998) Biochemistry 37, 13291.
Jin, W. et al. (1999) Biochemistry 38, 14294.
Drici, M.D. et al. (2000) Br. J. Pharmacol. 131, 569.
Kitamura, H. et al. (2000) Pharmacol. Exp. Ther. 293, 196.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Avoid exposure to light.

Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min). Avoid exposure to light. The product is lyophilized in 0.5 ml conical vial.

Storage of solutions Up to two weeks at 4°C or three months at -20°C. Avoid exposure to light.

Our bioassay

Alomone Labs Tertiapin-Q-ATTO Fluor-633 binds GIRK1/GIRK4-GFP transfected HEK293T cells.
Living cells were incubated in the presence of 100 nM Tertiapin-Q-ATTO Fluor-633 (#STT-170-FR). A. Visible light of cells. B. GIRK1/GIRK4-GFP transfected cells (green). C. Tertiapin-Q-ATTO Fluor-633 (red).
The pictures are a kind gift from the lab of Prof. Eithan Reuveny, Weizmann Institute, Israel.
Alomone Labs Tertiapin-Q-ATTO Fluor-633 inhibits K_ir3.2 channel heterologously expressed in Xenopus oocytes.
A continuous current trace recorded at a holding potential of -80 mV. K_ir3.2 currents are downward reflections activated by high K⁺containing solution. While activated, 50 nM and 100 nM of Tertiapin-Q-ATTO Fluor-633 (#STT-170-FR) were applied for 2 min (indicated as bars).

Scientific background

Tertiapin, the native toxin, was originally isolated from European honey bee Apis mellifera venom. Native and synthetic Tertiapin blocks a range of inward rectifier K⁺ channels (K_ir), in particular ROMK1 (K_ir1.1, IC₅₀= 2 nM) and GIRK (K_ir3 family, IC₅₀ for the K_ir3.1/3.4 heteromer was 8.6 nM) but with no effect on the K_ir2 family member¹. In accordance, it was shown to inhibit acetylcholine induced K⁺ currents in mammalian cardiomyocytes^2,3.

Tertiapin-Q is a derivative of Tertiapin in which Met¹³ is substituted by a Gln residue. However, unlike native Tertiapin, Tertiapin-Q is non-oxidizable and therefore is more stable⁴.

Tertiapin-Q inhibits the above-mentioned channels with similar affinities and also inhibits Ca²-activated large conductance BK-type K⁺ channels in a concentration and voltage-dependent manner⁵.

Target K_ir1.1, K_ir3.2 K⁺channels

Peptide Content: 100%

Lyophilized Powder

Last update: 02/01/2022

For research purposes only, not for human use

Specifications

Citations

Applications

Scientific Background

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Certificate of Analysis

Tertiapin-Q-ATTO Fluor-633

Specifications

Citations

Applications

Scientific Background

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