Anti-α1B-Adrenergic Receptor (extracellular)-ATTO-488 Antibody

Alpha-1B adrenoceptor, ADA1B, ADRA1B
Cat #: AAR-018-AG
Alternative Name Alpha-1B adrenoceptor, ADA1B, ADRA1B
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, r
      • Peptide (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human α1B-adrenoceptor (Accession P35368). Extracellular, N-terminus.
    Accession (Uniprot) Number P35368
    Gene ID 147
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 14/15 amino acid residues identical; rat - 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-018), and live cell imaging (labeled antibody).
    Applications: fc, ic, lci
    May also work in: ih
      • Direct flow cytometry analysis in rat GH3 pituitary adenoma cells:
        ___ Cells.
        ___ Cells + Anti-α1B-Adrenergic Receptor (extracellular)-ATTO-488 Antibody (#AAR-018-AG), (10 µg/5x10cells).
      • Expression of α1B-Adrenoceptor in rat GH3 cells
        Cell surface detection of α1B-Adrenoceptor in GH3 cells with Anti-α1B-Adrenergic Receptor (extracellular)-ATTO-488 Antibody (#AAR-018-AG) (green). Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342 (blue).
      • Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.

         
        Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

        Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2
         
        All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4
         
        α1B-Adrenoceptor populations are greatest in the spleen, kidney, cerebellum, and fetal brain.5

        α1B-Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 14/04/2019

    Anti-α1BAdrenergic Receptor (extracellular) Antibody (#AAR-018) is a highly specific antibody directed against an extracellular epitope of the human α1B-adrenoceptor. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize α1B-adrenoceptor from human, rat and mouse samples.

    Anti-α1B-Adrenergic Receptor (extracellular)-ATTO-488 Antibody (#AAR-018-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-α1B-Adrenergic Receptor (extracellular)-ATTO-488 Antibody has been tested in direct flow cytometry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use