Anti-Aquaporin 3-ATTO-594 Antibody

AQP3, Aquaglyceroporin-3, GIL, GLIP
Cat #: AQP-003-AR
Alternative Name AQP3, Aquaglyceroporin-3, GIL, GLIP
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3 (Accession P47862). Intracellular, C-terminus.
    Accession (Uniprot) Number P47862
    Gene ID 65133
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 17/18 amino acid residues identical; sheep, human - 15/18 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AQP-003), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic
      • Expression of Aquaporin 3 in rat colon
        Immunohistochemical staining of rat colon paraffin-embedded sections using Anti-Aquaporin 3-ATTO-594 Antibody (#AQP-003-AR), (1:100). Staining (red) is present in absorptive cells of the colonic epithelium. Hoechst 33342 is used as counterstain (blue).
        Expression of Aquaporin 3 in rat kidney
        Immunohistochemical staining of rat kidney paraffin-embedded sections using Anti-Aquaporin 3-ATTO-594 Antibody (#AQP-003-AR), (1:100). A. Staining (red) is present in collecting ducts in the corticomedullary junction. B. High magnification confocal image showing staining in discrete collecting ducts cells. Hoechst 33342  is used as counterstain (blue).
      • Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

        The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7AQP-9 and AQP-10.1 Little is known about the function of the two newest members, AQP-11 and AQP-12.  

        The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1

        AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 12/06/2019

    Anti-Aquaporin 3 Antibody (#AQP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize the AQP3 channel from rat, human, and mouse samples.

    Anti-Aquaporin 3-ATTO-594 Antibody (#AQP-003-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 3-ATTO-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use