Anti-Aquaporin 11 (extracellular) Antibody

AQP11
    Cat #: AQP-011
    Alternative Name AQP11
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
      • Peptide (C)SLSLTKYHFDER, corresponding to amino acid residues 140-151 of rat AQP11 (Accession Q8CHM1). 2nd extracellular loop.
        Anti-Aquaporin 11 (extracellular) Antibody
    Accession (Uniprot) Number Q8CHM1
    Gene ID 286758
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 11/12 amino acid residues identical.
    RRID AB_2341063.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
      • Anti-Aquaporin 11 (extracellular) Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
        1,2. Anti-Aquaporin 11 (extracellular) Antibody (#AQP-011), (1:1000).
        3,4. Anti-Aquaporin 11 (extracellular) Antibody, preincubated with the negative control antigen.
        Anti-Aquaporin 11 (extracellular) Antibody
        Western blot analysis of rat liver lysate:
        1. Anti-Aquaporin 11 (extracellular) Antibody (#AQP-011), (1:1000).
        2. Anti-Aquaporin 11 (extracellular) Antibody, preincubated with the negative control antigen.
    References
      • The aquaporins (AQP), also known as the major intrinsic protein superfamily (MIP), are a family of integral membrane proteins. In mammals, 13 aquaporins were described (AQP0–AQP12). Aquaporin 11 (AQP11) is a newly described member of the aquaporins family. Aquaporin 11 (AQP11) has been identified with unusual pore-forming NPA (asparagine-proline-alanine) boxes. AQP11 presents a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1. There are two subfamilies: the aquaporins, which transport only water and the aquaglyceroporins, which transport glycerol, urea, and other small solutes in addition to water2.

        Aquaporins and aquaglyceroporins are ubiquitously expressed, including in the plasma membrane of renal epithelia, brain astrocytes, and red blood cells3.  AQP11 is expressed in several tissues including testis, kidney, liver, and brain. In the brain, AQP11 is found in Purkinje cells, hippocampal neurons of CA1 and CA2, and cerebral cortical neurons4. In the kidney AQP11 has a central role in vesicular homeostasis as demonstrated in experiments using AQP11 knockout mice which display growth retardation and progressively die before weaning. The cause of death seems to be advanced renal failure5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Aquaporin 11. Anti-Aquaporin 11 (extracellular) Antibody (#AQP-011) can be used in western blot analysis. It has been designed to recognize AQP11 from rat and mouse samples.

    For research purposes only, not for human use