Anti-GABA(A) γ1 Receptor Antibody

GABRG1, Gamma-aminobutyric acid receptor subunit gamma-1

Cat #: AGA-016
Alternative Name GABRG1, Gamma-aminobutyric acid receptor subunit gamma-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A) γ1 receptor (Accession P23574). Intracellular, C-terminus.
Accession (Uniprot) Number P23574
Gene ID 140674
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 16/18 amino acid residues identical; human - 14/18 amino acid residues identical.
RRID AB_2340941.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), (1:500).
    3,4. Anti-GABA(A) γ1 Receptor Antibody, preincubated with GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016).
Immunohistochemistry
  • Expression of GABA(A) γ1 receptor in rat DRG
    Expression of GABA(A) γ1 receptor in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Staining (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
References
  1. Mihic, S.J. and Harris, R.A. (1994) Alcohol Health Res. World 21, 127.
  2. Whiting, P.J. et al. (1999) Neurochem. Int. 34, 387.
  3. Ashcroft, F.M. (2000) Ion Channels and Disease Ed 1.
  4. Mehta, A.K. and Ticku, M.K. (1999) Brain Res. Rev. 29, 196.
Scientific background

The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells1. There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors. GABAA R belongs to the ligand gated ion channel superfamily1,2.

The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity2,3.

They are composed of heteropentamer, with all of the five subunits contributing to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals.

mRNA of the γ1 subunit is detected in the amygdala and septum4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-GABA(A) γ1 Receptor Antibody (#AGA-016) is a highly specific antibody directed against an epitope of rat GABRG1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) γ1 from human, rat, and mouse samples.

For research purposes only, not for human use
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