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Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody

GABATR, GABT1, GABATHG, SLC6A1, Na+/Cl-- dependent GABA transporter 1
    Cat #: AGT-001
    Alternative Name GABATR, GABT1, GABATHG, SLC6A1, Na+/Cl-- dependent GABA transporter 1
  • Lyophilized Powder
  • Antigen Incl.
    • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1 (Accession P23978). 2nd  extracellular loop.
    • Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
    Accession (Uniprot) Number P23978
    Gene ID 79212
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_10917154.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
      1,2.  Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001), (1:200).
      3,4.  Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody
      Expression of GABATR in rat cerebellum
      Immunohistochemical staining of rat cerebellum using Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001), (1:200). A. GAT-1 staining is shown in green. B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse Anti-parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows). GAT-1 appears both in the soma of Purkinje cells (vertical arrows) and in the pinceau (horizontal arrows).
    References
    1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175.
    2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375.
    3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226.
    4. Conti, F. et al. (2011) Front. Cell. Neurosci. 5, 2.
    5. Lewis, D.A. et al. (2005) Nat. Rev. Neurosci. 6, 312.
    6. Fueta, Y. et al. (2003) Neuroscience 118, 371.
    7. Frahm, C. et al. (2004) NeuroReport 15, 9.
    Scientific background

    γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.

    To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1.

    GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4.

    GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized PowderNegative Control Antigen Included
    receive a 25 µl free trial sample!
    Last update: 30/01/2020

    Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAT-1 from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Rat brain lysate.
      Moldavan, M. et al. (2017) J. Neurophysiol. 118, 3092.
    Immunohistochemistry citations
    1. Human brain sections.
      Fuhrer, T.E. et al. (2017) Neuroscience 351, 108.

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