Anti-Gamma-Synuclein Antibody

γ-Synuclein, Breast Cancer-Specific Gene 1 Protein, BCSG1, Persyn, Synoretin, SNCG
  • New
Cat #: APZ-037
Alternative Name γ-Synuclein, Breast Cancer-Specific Gene 1 Protein, BCSG1, Persyn, Synoretin, SNCG
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)RKEDLEPPAQDQE, corresponding to amino acid residues 96 - 108 of rat γ-Synuclein (Accession O76070). Intracellular, C-terminus.
    • Anti-Gamma-Synuclein Antibody
    Accession (Uniprot) Number O76070
    Gene ID 64347
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Specificity Not recommended for use with human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*
    Western blot
    • Anti-Gamma-Synuclein Antibody
      Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
      1, 2. Anti-Gamma-Synuclein Antibody (#APZ-037), (1:200).
      3, 4. Anti-Gamma-Synuclein Antibody, preincubated with the negative control antigen.
    References
    1. George, J.M. (2002) Genome Biol. 3, 3002.1.
    2. Ji, H. et al. (1997) Cancer Res. 57, 759.
    3. Lavedan, C. et al. (1998) Hum. Genet. 103, 106.
    4. Duda, J.E. et al. (1999) Exp. Neurol. 160, 515.
    5. Jia, T. et al. (1999) Cancer Res. 59, 742.
    Scientific background

    γ-synuclein is a 127 amino acids protein that belongs to the synuclein family of proteins. Synucleins are natively unfolded proteins, i.e. they have no secondary or tertiary structure in solution and hence these proteins are heat stable. The unusual biophysical properties of synucleins are rooted in their characteristic primary sequence that includes a highly conserved N-terminal domain with an 11- residue repeat and a less conserved acidic C-terminal domain that contains many proline residues1.

    Mammalian γ-synuclein, encoded by BCSG1 (breast cancer specific gene 1), was discovered in a screen for breast cancer markers2. It is expressed in the peripheral nervous system in primary sensory neurons, sympathetic neurons, and motor neurons. It is also detected in the brain3, olfactory epithelium and breast/ovarian tumors4. Indeed, exogenous expression of this protein increases the invasive and metastatic potential of breast tumors5.

    The roles of synucleins are not fully elucidated but this group of conserved and unusual proteins contributes to diverse disease processes spanning neurodegenerative disease and cancer and hence, studying the synucleins specific cellular activity is very tempting and promising.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 15/06/2020

    Anti-Gamma-Synuclein Antibody (#APZ-037) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot analysis. It has been designed to recognize γ-Synuclein from mouse and rat samples. The antibody will not recognize the protein from human samples.

    For research purposes only, not for human use
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