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Peptide (C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat Glucose transporter 1 (Accession P11167). 1st extracellular loop.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GLUT1 (extracellular) Antibody (#AGT-041), (1:400).
3,4. Anti-GLUT1 (extracellular) Antibody, preincubated with the control peptide antigen.Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 5), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 6), human K562 chronic myeloid leukemia cell line lysate (lanes 3 and 7) and human Jurkat T-cell leukemia cell line lysate (lanes 4 and 8):1-4. Anti-GLUT1 (extracellular) Antibody (#AGT-041), (1:400).
5-8. Anti-GLUT1 (extracellular) Antibody, preincubated with the control peptide antigen.
Indirect flow cytometry of live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-GLUT1 (extracellular) Antibody (#AGT-041), (5 µg) + goat-anti-rabbit-FITC.
Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS). It is encoded by SLC2A1, and mediates basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.
GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.
Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.
Species reactivity key:
Anti-GLUT1 (extracellular) Antibody (#AGT-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GLUT1 from human, rat, and mouse samples.