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Anti-Human Orai1 (extracellular)-FITC Antibody

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
Cat #: ACC-060-F
Alternative Name TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31). 2nd extracellular loop.
Accession (Uniprot) Number Q96D31
Gene ID 84876
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Canis - identical; bovine - 12/13 amino acid residues.
RRID AB_2039845.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will not recognize mouse or rat Orai1.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-060), and direct flow cytometry analysis (labeled antibody).
Applications: fc
Direct flow cytometry
  • Cell surface detection of Orai1 in live Jurkat (human acute T cell leukaemia) cells:
    Cell surface detection of Orai1 in live Jurkat (human acute T cell leukaemia) cells:
    ___ Unstained cells.
    ___ Cells + Anti-Human Orai1 (extracellular)-FITC Antibody (#ACC-060-F), (10 µg/1x106 cells).
  • The control antigen is not suitable for this application.
References
  1. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3.
  2. Chakrabarti, R. and Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503.
  3. Feske, S. et al. (2006) Nature 441, 179.
  4. Pickett, J. (2006) Nature Reviews Mol. Cell Biol. 7, 794.
  5. Luik, R.M. et al. (2006) J.Cell. Biol. 174, 815.
  6. Wu, M.M. et al. (2006) J.Cell. Biol. 174, 803.
  7. Hong H.L. et al. (2007) J. Biol. Chem. 282, 9105.
  8. Luik, R.M and Lewis, R.S. (2007) Trends Mol. Med. 13, 103.
  9. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282, 17548.
  10. Lis, A. et al. (2007) Curr. Biol. 17, 794.
Scientific background

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.

Recently, several key players of the store operated complex have been identified3Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.

Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9,10.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-Human Orai1 (extracellular) Antibody (#ACC-060) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize Orai1 from human samples only.

Anti-Human Orai1 (extracellular)-FITC Antibody (#ACC-060-F) is directly conjugated to FITC. This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

For research purposes only, not for human use
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