Free shipping starts now, no minimum, no coupons required!

Anti-MANF/ARMET Antibody

Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP
Cat #: ANT-028
Alternative Name Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF (Accession P55145). Mature MANF protein.
Accession (Uniprot) Number P55145
Gene ID 7873
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - identical.
RRID AB_2756712.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human recombinant MANF protein (lanes 3 and 6):
    Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human recombinant MANF protein (lanes 3 and 6):
    1-3. Anti-MANF/ARMET Antibody (#ANT-028), (1:200).
    4-6. Anti-MANF/ARMET Antibody, preincubated with MANF/ARMET Blocking Peptide (#BLP-NT028).
Immunohistochemistry
  • Expression of MANF in rat parietal cortex
    Expression of MANF in rat parietal cortex
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-MANF/ARMET Antibody (#ANT-028), (1:600), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Sections derived from parietal cortex of a rat at postnatal day 22 reveal MANF staining (green) in nuclei and cytoplasm of several cells (arrows) in deep layers of the cortex. Nuclei are stained with DAPI (blue).
References
  1. Glembotski, C.C. (2011) J. Mol. Cell. Cardiol. 51, 512.
  2. Lindholm, P. and Saarma, M. (2010) Dev. Neurobiol. 70, 360.
  3. Parkash, V. et al. (2009) Protein Eng. Des. Sel. 22, 233.
  4. Airavaara, M. et al. (2012) Parkinsonism Relat. Disord. 18, S143.
  5. Lindholm, P. et al. (2008) Mol. Cell. Neurosci. 39, 356.
  6. Allen, S.J. et al. (2013) Pharmacol. Ther. 138, 155.
  7. Wang, H. et al. (2014) PLoS One 9, e90433.
Scientific background

The novel protein mesencephalic astrocyte-derived neurotrophic factor (MANF) is a survival molecule (alternatively referred to as arginine-rich, mutated in early stage tumors, or ARMET) that has high selectivity for dopaminergic neurons of the midbrain1,2. There, it induces proliferative and protective effects against neurodegeneration (usually caused by 6-hydroxydopamine); its therapeutic potential in dopamine-related neurodegenerative disorders such as Parkinson's disease is therefore promising3,4.

MANF is also expressed cardiac tissues. As was recently suggested, it induces cardioprotection in the heart and anti-hypertrophic effects via reducing endoplasmic reticulum stress in ischemic conditions in both the heart and the brain. Thus, its effects stretch beyond rescue and protection of dopaminergic neurons alone1,5.

Generally, neurotrophic factors (NTFs) provide support, protection, and regulation for neurons – with each group of neurons having their own unique NTFs – both during development and at adulthood. Other noticeable NTFs include neurotrophin-3 and -4 (NT3 and NT4), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and glial cell derived neurotrophic factor (GDNF)6.

MANF is considered to be evolutionary conserved within Mammalia and beyond (including distant species such as nematode and fruit fly – the latter shares more than 50% identity with human MANF gene) and is highly mutative in many tumors. It is mostly composed of alpha helices and random coils; its structure essentially differs from any other neurotrophic factor. It is suggested that the protein initiates intracellular signaling cascade by binding to cell-surface lipids and membranes via its cysteine-rich N-terminal. Together with its C-terminal which contains a disulfide bridge that endows a cytoprotective response against ER stress, MANF is implied to be bi-functional3,7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Anti-MANF/ARMET Antibody (#ANT-028) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MANF from human, rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information