Anti-SCN3A (NaV1.3) (extracellular) Antibody

BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha
    Cat #: ASC-023
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: r
    May also work in: h, m
    Immunogen
    Peptide CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3 (Accession P08104). 3rd extracellular loop, domain I.
    Accession (Uniprot) Number P08104
    Gene ID 497770
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – 11/12 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity May recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 2 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat newborn brain lysates (lanes 1 and 3), rat adult brain membranes (lane 2):
    1,2. Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023), (1:200).
    3. Anti-SCN3A (NaV1.3) (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of NaV1.3 in HEK-293 transfected cells
    Immunocytochemical staining of intact living HEK-293 cells expressing rat NaV1.3. A. Extracellular staining of cells using Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023), (red). B. Cells transfected with the empty vector show no NaV1.3 staining. Nuclear staining using DAPI as the counterstain (blue).
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, X. et al. (2002) J. Neurosci22, 7425.
    3. Fjell, J. et al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. et al. (2001) Trends. Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist 7, 42.
    7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
    8. Lai, J. et al. (2004) Annu. Rev. Pharmacol. Toxicol. 44, 371.
    9. Hains, B.C. et al. (2003) J. Neurosci. 23, 8881.
    10. Cummins, T.R. et al. (2001) J. Neurosci. 21, 5952.
    11. Shah, B.S. et al. (2001) J. Physiol. 534, 763.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5  The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3

    Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7
     
    NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 29/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat NaV1.3 channel. Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023) can be used in western blot, and live cell imaging applications. It has been designed to recognize NaV1.3 from rat, human and mouse samples. The antibody may recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-SCN3A (NaV1.3) Antibody (#ASC-004). This antibody recognizes a different epitope and thus can also be used as a control for detecting NaV1.3.