Tetrodotoxin citrate free

TTX
A Classic Blocker of a Subset of NaV Channels
    Cat #: T-500
  • Lyophilized Powder
  • Bioassay Tested
  • Shipped at Room Temp.
  • Origin Tetraodon pardalis (puffer fish).
    Source Natural
    MW: 319.28
    Purity: >98%
    Effective concentration 100 nM - 1 µM.
    Structure
    Chemical name Octahydro-12-(hydroxymethyl)-2-imino-5,9:7,10adimethano-10aH-[1,3]dioxocino[6,5-d]pyrimidine-4,7,10,11,12-pentol.
    Molecular formula C11H17N3O8.
    CAS No.: 4368-28-9.
    Activity Tetrodotoxin is a potent and selective blocker of a subclass of NaV channels1, and is often used to define subclasses of NaV channels2.
    Storage before reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at 4°C.
    Reconstitution Acidic buffer (pH 4.8). Centrifuge all product preparations before use (10000 x g 5 min).
    Storage after reconstitution Up to one week at 4°C or six months at -20°C.
    Our bioassay
    Alomone Labs Tetrodotoxin citrate free inhibits native NaV currents in PC-12 and ND7-23 cells.
    Tetrodotoxin citrate free (#T-500) inhibits PC-12 (top) and ND7-23 (bottom) cells at a concentration of 60 nM. Left graphs show the time course of inward current amplitude upon bath application (marked by the horizontal bars) and wash of Tetrodotoxin citrate free. Membrane potential was clamped to -120 mV and currents were elicited by 20 ms test pulses to -10 or -20 mV (upper and lower graphs respectively), delivered every 10 sec. Right graphs show the superimposed traces of control current (red) and during perfusion of Tetrodotoxin citrate free (black).
    References
    1. Narahashi, Tet al. (1964) J. Gen. Physiol47, 965.
    2. Hille, B. (2001) In: Ion Channels in Excitable Membranes (third edition) chapter 3.
    Scientific background Tetrodotoxin (TTX) is an established potent and selective blocker of some NaV channels.1 Moreover, TTX sensitivity is used to define subclasses of NaV channels.2 It is widely used both to probe NaV activity and to reduce or block excitability in neurophysiological preparations.
    Target Various NaV Na+ channels

    Promotion

    Use code: ttx-30-1718

    Tetrodotoxin promotion

    Last update: 31/07/2018

    Tetrodotoxin citrate free (#T-500) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use
    Citations
    Product citations
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    2. Bant, J.S. et al. (2013) J. Neurosci. 33, 4976.
    3. Browning, K.N. et al. (2013) J. Physiol. 591, 1563.
    4. Browning, K.N. et al. (2013) J. Physiol. 591, 2357.
    5. Dvorzhak, A. et al. (2013) J. Physiol. 591, 1145.
    6. Lu, V.B. et al. (2013) Mol. Pharmacol. 83, 267.
    7. Luo, R. et al. (2013) J. Physiol. 591, 203.
    8. Perez, G.J. et al. (2013) Am. J. Physiol. 304, C280.
    9. Ran, I. et al. (2013) J. Neurosci. 33, 1872.
    10. Ryan, R.T. et al. (2013) Cereb. Cortex 23, 1198.
    11. Tonini, R. et al. (2013) J. Neurophysiol. 109, 1514.
    12. Wen, H. et al. (2013) J. Neurosci. 33, 7384.
    13. Akemann, W. et al. (2012) J. Neurophysiol. 108, 2323.
    14. Colgan, L.A. et al. (2012) J. Neurosci. 32, 15737.
    15. Gkogkas, C.G. et al. (2012) Nature 493, 371.
    16. Goutman, J.D. (2012) J. Neurosci. 32, 17025.
    17. Janiek, R. et al. (2012) J. Physiol. 590, 5091.
    18. Kohlmeier, K.A. et al. (2012) J. Neurophysiol. 108, 2751.
    19. Song, W. et al. (2012) J. Physiol. 590, 5123.
    20. Guzman, J.N. et al. (2009) J. Neurosci. 29, 11011.
    21. Wan, S. et al. (2008) J. Neurosci. 28, 4957.
    22. Hirasawa, M. et al. (2004) J. Physiol. 559, 611.
    23. Jennings, E.A. et al. (2001) J. physiol. 534, 805.
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