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Anti-CD56/NCAM1 (extracellular) Antibody

Neural cell adhesion molecule 1, NCAM-1

Cat #: ANR-041
Alternative Name Neural cell adhesion molecule 1, NCAM-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1 (Accession P13596). Extracellular, N-terminus.
Accession (Uniprot) Number P13596
Gene ID 24586
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/15 amino acid residues identical.
RRID AB_2756690.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of mouse brain (lanes 1 and 6), rat cerebellum (lanes 2 and 7), rat eye (lanes 3 and 8), SH-SY5Y (lanes 4 and 9) and PC12 (lanes 5 and 10) lysates:
    Western blot analysis of mouse brain (lanes 1 and 6), rat cerebellum (lanes 2 and 7), rat eye (lanes 3 and 8), SH-SY5Y (lanes 4 and 9) and PC12 (lanes 5 and 10) lysates:
    1-5. Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (lanes 1-3 1:400 and lanes 4-5 1:200).
    6-10. Anti-CD56/NCAM1 (extracellular) Antibody, preincubated with CD56/NCAM1 (extracellular) Blocking Peptide (#BLP-NR041).
Immunohistochemistry
  • Expression of NCAM1 in rat olfactory bulb
    Expression of NCAM1 in rat olfactory bulb
    Immunohistochemical staining of rat olfactory bulb frozen section using Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (1:300). A. NCAM1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). B. Nuclei are stained using DAPI as the counterstain. C. Merged image of A and B panels.
Live cell imaging / Immunocytochemistry
  • Expression of NCAM1 in rat PC12 cells
    Expression of NCAM1 in rat PC12 cells
    Cell surface detection of NCAM1 in intact living rat pheochromocytoma (PC12) cells using Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (1:100). A, B. Extracellular staining of cells merged with a live view of the cells.
References
  1. Johnson, C.P. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 6963.
  2. Lanier, L.L. et al. (1991) J. Immunol. 146, 4421.
  3. Kiss, J.Z. and Muller, D. (2001) Rev. Neurosci. 12, 297.
  4. Arai, M. et al. (2004) Biol. Psychiatry 55, 804.
  5. Ćirović, S. et al. (2014) Int. J. Clin. Exp. Pathol. 7, 1402.
Scientific background

Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3

NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4.

Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041) is a highly specific antibody directed against rat Neural cell adhesion molecule 1. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NCAM1 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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