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Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody

Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304

Cat #: ANR-063-F
Alternative Name Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GGKHRENKVFMRK, corresponding to amino acid residues 502-514 of human neuropilin-1 (Accession O14786). Extracellular, N-terminus.
Accession (Uniprot) Number O14786
Gene ID 8829
Homology Rat, mouse - identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (Unlabeled antibody, #ANR-063), and direct flow cytometry (Labeled antibody).
Applications: fc
Direct flow cytometry
  • Cell surface detection of Neuropilin-1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of Neuropilin-1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody (#ANR-063-F), (2.5µg).
  • Cell surface detection of Neuropilin-1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Neuropilin-1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti- Neuropilin-1 (NRP1) (extracellular)-FITC Antibody (#ANR-063-F), (2.5µg).
References
  1. Pellet-Many, C. et al. (2008) Biochem. J. 411, 211.
Scientific background

Neuropilins (NRPs) are co-receptors for class 3 semaphorins, polypeptides with key roles in axonal guidance, and for members of the VEGF (vascular endothelial growth factor) family of angiogenic cytokines.

Neuropilin 1 (NRP1) is a transmembrane glycoprotein and comprises a large extracellular region containing multiple domains, a single transmembrane domain and a small cytoplasmic domain, also with a number of protein interacting domains.

Class 3 semaphorins bind NRP1 (and NRP2) with different, and partially overlapping, specificities and affinities. Plexin A1 and Plexin A2 are the major signaling receptors for class 3 semaphorins, and complex with NRPs and semaphorins to form a holoreceptor essential for mediating effects of semaphorins on growth-cone collapse and axonal guidance in neurogenesis. The major VEGF-A signaling receptor, VEGF receptor 2, forms complexes with NRP1 and is important for optimal VEGFR2 signaling and function in endothelial cells.

Targeted disruption of the NRP1 gene has demonstrated an essential dual role of these molecules in neurogenesis and cardiovascular development. NRP1-null mice die with a spectrum of cardiovascular and neuronal defects1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 03/03/2021

Anti-Neuropilin-1 (NRP1) (extracellular) Antibody (#ANR-063) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and live cell flow cytometry applications. It has been designed to recognize NRP1 from human, mouse, and rat samples.

Anti-Neuropilin-1 (NRP1)-FITC Antibody (#ANR-063-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use
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