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Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody

P2RX1, P2X purinoceptor 1

Cat #: APR-022-AG
Alternative Name P2RX1, P2X purinoceptor 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575). Extracellular loop.
Accession (Uniprot) Number P51575
Gene ID 5023
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 11/14 amino acid residues identical.
RRID AB_2341049.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-022), and immunohistochemistry (labeled antibody).
Applications: ic, if, ih, lci
Immunohistochemistry
  • Expression of P2X1 receptor in mouse cerebellum
    Expression of P2X1 receptor in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-022-AG). A. Most of P2RX1 labeling (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells. B. Nuclear staining using DAPI. C. Merge of panels A and B.
Live cell imaging / Immunocytochemistry
  • Expression of P2X1 receptor in rat PC12 cells
    Expression of P2X1 receptor in rat PC12 cells
    Cell surface detection of P2X1 receptor in intact living PC12 cells. A. Extracellular staining of cells using Anti-P2X1 receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-022-AG), (green), (1:20). B. Merge of extracellular staining with live view of the cells.
References
  1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
  2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
  3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
  4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
  5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
  6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
  7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
  8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
  9. Kim, M. et al. (2001) EMBO J. 20, 6347.
  10. Burnstock, G. (2001) Trends Pharmacol. Sci. 22, 182.
  11. Oury, C. et al. (2003) Blood 101, 3969.
Scientific background

The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned1-3. All P2X receptor subtypes share the same structure of intracellular N- and C-termini two membrane-spanning domains and a large extracellular loop.

All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex4-9.

The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous systems, while the P2X7 receptor is predominantly found in cells of the immune system4. The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis3,4,10,11.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 08/06/2022

Anti-P2X1 Receptor (extracellular) Antibody (#APR-022) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize P2X1 receptor from rat, mouse, and human samples.

Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-022-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

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For research purposes only, not for human use
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