Free shipping starts now, no minimum, no coupons required!

Anti-P2X3 Receptor Antibody

P2RX3, P2X purinoceptor 3

Cat #: APR-016
Alternative Name P2RX3, P2X purinoceptor 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor (Accession P49654). Intracellular, C-terminus.
Accession (Uniprot) Number P49654
Gene ID 81739
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/15 amino acid residues identical.
RRID AB_2313760.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, wb
May also work in: ip*
Western blot
  • Western blot analysis of rat DRG lysates:
    Western blot analysis of rat DRG lysates:
    1. Anti-P2X3 Receptor Antibody (APR-016), (1:200).
    2. Anti-P2X3 Receptor Antibody, preincubated with P2X3 Receptor Blocking Peptide (#BLP-PR016).
Immunohistochemistry
  • Expression of P2X3 Receptor in rat DRG neurons
    Expression of P2X3 Receptor in rat DRG neurons
    Immunohistochemical staining of rat dorsal root ganglion (DRG) neurons with Anti-P2X3 Receptor Antibody (#APR-016), (A). Cells within the DRG were stained (see solid line frame enlarged (B) as well as fibers and the area of entry of dorsal root into spinal cord (see dashed line frame enlarged (C). DAPI is used as the counterstain (in B and C).
Immunocytochemistry
  • Fixed mouse trigeminal neurons (1:200) (D'Arco, M. et al. (2007) J. Neurosci. 27, 8190.).
Indirect flow cytometry
  • Human CD4+ and ckit+ cells (Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.).
References
  1. Florenzano, F. et al. (2002) Neuroscience 115, 425.
  2. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed.1.
  3. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
  4. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
  5. Kage, K. et al. (2002) Exp. Brain Res. 147, 511.
  6. Prasad, M. et al. (2001) J. Physiol. 573, 667.
  7. Aoki, Y. et al. (2003) Brain Res. 989, 214.
  8. Burnstock, G. (2000) Br. J. Anaesth. 84, 476.
Scientific background

The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3

All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-P2X3 Receptor Antibody (#APR-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat DRG lysate (1:1000).
    Chen, Y. et al. (2016) Mol. Pain 11, 1.
  2. Mouse neuron lysate (1:300).
    Gnanasekaran, A. et al. (2013) J. Neurochem. 126, 102.
Immunohistochemistry citations
  1. Mouse geniculate ganglion (1:500).
    Larson, E.D. et al. (2020) eNeuro 7, 0339-19.2020.
  2. Mouse tongue sections (1:300).
    Voigt, A. et al. (2015) J. Neurosci. 35, 9717.
Immunocytochemistry citations
  1. Human ESC NPs (1:100).
    Forostyak, O. et al. (2013) Stem Cells Dev. 22, 1506.
  2. Mouse trigeminal neurons (1:200).
    Gnanasekaran, A. et al. (2013) J. Neurochem. 126, 102.
  3. Fixed mouse trigeminal neurons (1:200).
    D'Arco, M. et al. (2007) J. Neurosci. 27, 8190.
Immunofluorescence citations
  1. Mouse geniculate ganglion (1:500).
    Larson, E.D. et al. (2020) eNeuro 7, 0339-19.2020.
Indirect flow cytometry citations
  1. Human CD4+ and ckit+ cells.
    Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.
More product citations
  1. Franceschini, A. et al. (2012) BMC Neurosci. doi:10.1186/1471-2202-13-143.
Shipping and Ordering information