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Home › Products › Ion Channels › Ligand-Gated Ion Channels › Purinergic (P2X) Receptors › Antibodies to P2X Receptors

Certificate of Analysis

Anti-P2X3 Receptor (extracellular) Antibody

P2RX3, P2X purinoceptor 3

Cat #: APR-026
Alternative Name P2RX3, P2X purinoceptor 3
  • KO Validated
    The specificity of this antibody has been validated in a knockout (KO) or knockdown (KD) system.
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
    Accession (Uniprot) Number P49654
    Gene ID 81739
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human - 13/15 amino acid residues identical.
    RRID AB_2341052.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, lci, wb
    May also work in: ip*
    Western blot
    • Rat DRG lysate (1:200).
    • Western blot analysis of rat DRG:
      Western blot analysis of rat DRG:
      1. Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (1:200).
      2. Anti-P2X3 Receptor (extracellular) Antibody, preincubated with P2X3 Receptor (extracellular) Blocking Peptide (#BLP-PR026).
    Immunohistochemistry
    • Rat dorsal root ganglion (DRG).
    Indirect flow cytometry
    • Cell surface detection of P2X3 Receptor by indirect flow cytometry in live intact mouse P815 mastocytoma cells:
      Cell surface detection of P2X3 Receptor by indirect flow cytometry in live intact mouse P815 mastocytoma cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells +  Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (2.5μg) + goat-anti-rabbit-FITC.
    Live cell imaging / Immunocytochemistry
    • Rat pheochromocytoma (PC12) cells.
    Scientific background

    The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3

    All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized Powder
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    Last update: 11/04/2021

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    For research purposes only, not for human use

    Specifications

    Applications

    Scientific Background

    Citations

    Citations
    Shipping and Ordering information
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      Control Product

      Save $45 off the Blocking Peptide when purchased together with the antibody
      • A Blocking peptide for Anti-P2X3 Receptor (extracellular) Antibody (#APR-026). Intended to be used as a negative control.


        Price: $245

        P2X3 Receptor (extracellular) Blocking Peptide (#BLP-PR026)
        Add peptide to cart

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