- Peptide (C)RKDEKTAQTRESHAK, corresponding to amino acid residues 161-175 of mouse Pacsin3 (Accession Q99JB8). Intracellular.
- Western blot analysis of rat brain membrane (lanes 1 and 5), rat skeletal muscle lysate (lanes 2 and 6), rat heart lysate (lanes 3 and 7) and mouse heart membrane (lanes 4 and 8):1-4. Anti-PACSIN3 Antibody (#AIP-015), (1:400).
5-8. Anti-PACSIN3 Antibody, preincubated with PACSIN3 Blocking Peptide (#BLP-IP015).
Pacsin 3 (also known as Syndapin 3) is a member of the highly conserved Pacsin protein sub-family which constitutes a branch of the larger F-BAR domain protein family.
Pacsin 3, as well as other members of its family, is a cytoplasmic protein involved in receptor-mediated endocytosis, synaptic vesicle trafficking and biogenesis of different cellular organelles and is mostly, but not only, expressed in lung and muscle tissues. Pacsin 3 contains an N-terminal F-BAR domain and a C-terminal mono-Src homology 3 domain1.
The C-terminal SH3 domain of Pacsin 3 interacts with the N-terminal proline-rich domain of TRPV4 at residues 132-144 and enhances the cell surface expression of TRPV4, probably through the inhibition of the endocytotic process2. The SH3 domain is also responsible for interactions with the protein dynamin and with N-WASP, a potent activator of the Arp2/3 complex F-actin-nucleation machine3. Pacsin’s interaction with dynamin contributes to fusion pore expansion in chromaffin cells in the adrenal medulla. Pacsin-dynamin interaction occurs through an SH3-PRD binding interaction that depends on the central proline residue in the ligand-binding site of Pacsin’s SH3 domain4.
Pacsin 3 is also involved in the trafficking of AMPA receptors in neurons through its interaction with PICK1 (Protein interacting with C-kinase 1). PICK1 and Pacsin 3 form a complex with the C-termini of the AMPA subunits GluA2 and GluA3. This interaction between Pacsin 3 and PICK1 is regulated by Pacsin phosphorylation and is required for NMDA induced AMPA receptor endocytosis and cerebellar long term synaptic depression5.
Species reactivity key:
Anti-PACSIN3 Antibody (#AIP-015) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Pacsin 3 from rat, mouse, and human samples.