Mouse NGF Deluxe Research Pack

All You Need for NGF Research
  • Lyophilized Powder
  • Antigen Incl.
Cat #: ESD-651
Sizes: 14 Vials
Last update: 21/11/2019

Alomone Labs is pleased to offer the Mouse NGF Deluxe Research Pack (#ESD-651). The Research Pack contains all you need for human NGF research: Antibodies to NGF and its receptors, natural NGF and a specific TrkA receptor inhibitor all in one economical package!

For research purposes only, not for human use
Compounds
Product NameCat #Size
Antibodies
Anti-NGF Antibody
AN-240 1 x 0.2 ml
Mouse NGF/proNGF Neutralizing Antibody
ALM-006 1 x 0.25 mg
Anti-p75 NGF Receptor (extracellular) Antibody
ANT-007 1 x 0.2 ml
Anti-p75 NGF Receptor (extracellular)-ATTO-488 Antibody
ANT-007-AG 1 x 50 µl
Anti-TrkA (extracellular) Antibody
ANT-018 1 x 0.2 ml
Anti-TrkA (extracellular)-ATTO-633 Antibody
ANT-018-FR 1 x 50 µl
Blockers/Antagonists
K252a
K-150 1 x 0.1 mg
Modulators
GW441756 hydrochloride
G-191 1 x 25 mg
Proteins
Native mouse NGF 2.5S protein (>95%)
N-100 1 x 0.1 mg
Note A mouse monoclonal antibody (#ALM-006) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
Scientific Background
    • The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4 

      NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid  
      chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse. 

      NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11  

      The biological effects of NGF are mediated by two receptors: 
      TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12

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