Certificate of Analysis

Shaker (KV)-Related Channel Antibody Explorer Kit

A Screening Package of Shaker (KV)-Related Channel Antibodies Economically Priced
Cat #: AK-220 36 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-DPP6 (extracellular) Antibody Cat #: APC-146

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KKHEDESEAWLHR, corresponding to amino acid residues 414-426 of human DPP6 (Accession P42658). Extracellular, C-terminus.
Homology
Rat, mouse - identical.

Anti-DPP10 (extracellular) Antibody Cat #: APC-147

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CSRKYEMTSDTWISK, corresponding to amino acid residues 356-370 of rat DPP10 (Accession Q6Q629). Extracellular, C-terminus.
Homology
Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.

Anti-KV2.1 Antibody Cat #: APC-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (CY)HMLPGGGAHGSTRDQSI, corresponding to amino acid residues 841-857 of rat KV2.1 (Accession P15387). Intracellular, C-terminus.
Homology
Mouse - identical; human, rabbit - 15/17 amino acid residues identical.

Guinea pig Anti-KV2.1 Antibody Cat #: AGP-109

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)HMLPGGGAHGSTRDQSI, corresponding to amino acid residues 841-857 of rat KV2.1 (Accession P15387). Intracellular, C-terminus.
Homology
Mouse - identical; human - 15/17 amino acid residues identical.

Anti-KV2.2 (KCNB2) Antibody Cat #: APC-120

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CRQDIYQAVGEVKKD, corresponding to amino acid residues 859-873 of rat KV2.2 (Accession Q63099). Intracellular, C-terminus.
Homology
Mouse - identical; human - 13/15 amino acid residues identical.

Anti-KV3.1b (KCNC1) Antibody Cat #: APC-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CKESPVIAKYMPTEAVRVT, corresponding to amino acid residues 567-585 of rat KV3.1b (Accession P25122). Intracellular, C-terminus.
Homology
Mouse, human, dog – identical.

Anti-KV3.2 (KCNC2) Antibody Cat #: APC-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide DLGGKRLGIEDAAGLGGPDGK(C), corresponding to amino acid residues 184-204 of rat KV3.2 (Accession P22462). Intracellular, N-terminal part.
Homology
Human - 19/21 amino acid residues identical (based on ESTs).

Anti-KCNC3 (KV3.3) Antibody Cat #: APC-102

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide KSPITPGSRGRYSRDRAC, corresponding to amino acid residues 701-718 of rat KCNC3 (Accession Q01956). Intracellular, C-terminus.
Homology
Human, mouse-identical.

Anti-KCNC4 (KV3.4) Antibody Cat #: APC-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide EAGDD ERELA LQRLG PHEG(C), corresponding to amino acid residues 177-195 of rat KCNC4 (Accession Q63734). Intracellular, N-terminal.
Homology
Human - identical.

Anti-KV4.1 (KCND1) Antibody Cat #: APC-119

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KRRAIRLANSTAS, corresponding to amino acid residues 538-550 of human KCND1 (Accession Q9NSA2). Intracellular, C-terminal domain.
Homology
Mouse, rat - 12/13 amino acid residues identical.

Anti-KV4.2 Antibody Cat #: APC-023

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2 (Accession Q63881). Intracellular, C-terminus.
Homology
Mouse, human - 15/16 amino acid residues identical.

Guinea pig Anti-KV4.2 Antibody Cat #: AGP-038

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2 (Accession Q63881). Intracellular, C-terminus.
Homology
Mouse, human - 15/16 amino acid residues identical.

Anti-KV4.3 Antibody Cat #: APC-017

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)NEALELTGTPEEEHMGK, corresponding to amino acid residues 451-468 of human KV4.3 (Accession O60577). Intracellular, C-terminus.
Homology
Rat, rabbit - identical; mouse - 16/17 amino acid residues identical.

Anti-KChIP1 (KCNIP1) Antibody Cat #: APC-141

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)SLQTKQRRPSKD, corresponding to amino acid residues 10-21 of rat KChIP1 (Accession Q8R426). N-terminal part.
Homology
Mouse, human - identical.

Anti-KChIP2 Antibody Cat #: APC-142

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)RGQGRKESLSDSRD, corresponding to amino acid residues 2-15 of human KChIP2 (Accession Q9NS61). Cytoplasmic, N-terminus.
Homology
Mouse - identical. Rat - 13/14 amino acid residues identical.

Anti-KChIP3 (CSEN, DREAM) Antibody Cat #: APC-143

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)SKREGIKWQRPR, corresponding to amino acid residues 18-31 of rat KChIP3 (Accession Q9JM47). Cytoplasmic, N-terminus.
Homology
Human, mouse - 11/12 amino acid residues identical.

Anti-KCNE4 (MiRP3) Antibody Cat #: APC-129

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)GYMKSKRREKKSS, corresponding to amino acid residues 56-68 of mouse KCNE4 (Accession Q9WTW3). Intracellular, C-terminus.
Homology
Rat, human – identical.

Guinea pig Anti-Connexin-43 Antibody Cat #: AGP-141

Size: 50 µl Host: Guinea Pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus.
Homology
Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Guinea pig polyclonal antibodies (#AGP-038 & #AGP-109) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.