Overview
- Peptide (C)NGSVGSEDQQLEPAHIS, corresponding to amino acid residues 39-55 of rat κ-Opioid receptor (Accession P34975). Extracellular, N-terminus.
Cell surface detection of Kappa Opioid Receptor by direct flow cytometry in live intact mouse BV-2 microglia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-κ-Opioid Receptor (extracellular)-FITC Antibody (#AOR-012-F), (5µg).
Cell surface detection of Kappa Opioid Receptor by direct flow cytometry in live intact mouse J774 macrophage cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-κ-Opioid Receptor (extracellular)-FITC Antibody (#AOR-012-F), (5µg).
- Pan, L. et al. (2005) Neuroscience 133, 209.
- IUPHAR
- Matthes, H.W. et al. (1998) J. Neurosci. 18, 7285.
- Di Chiara, G. and North, R.A. (1992) Trends Pharmacol. Sci. 13, 185.
- Schwarzer, C. (2009) Pharmacol. Ther. 123, 353.
Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor1. Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors2.
The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins1.
All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-opioid receptor, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level3. The opioid receptors also mediate the mood-altering properties of opioids4.
κ-Opioid Receptor is activated by the endogenous peptide dynorphin. Malfunction of their signaling may be involved in epilepsy, addiction, depression, schizophrenia, and chronic pain5.
κ-Opioid Receptor is localized to a number of regions in the brain, as well as the spinal cord, the enteric nervous system and the digestive system5.
Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody (#AOR-012) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize κ-Opioid Receptor from rat, mouse and human samples.
Anti-κ-Opioid Receptor (extracellular)-FITC Antibody (#AOR-012-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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