Anti-α1-Syntrophin Antibody

Syntrophin-1, 59 KDa Dystrophin-Associated Protein A1 Acidic Component 1, SNTA1
    Cat #: APZ-021
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RQPSSPGPQPRNLSE, corresponding to amino acid residues 191-205 of mouse α1-Syntrophin (Accession Q61234). Intracellular, PH1 domain.
    Accession (Uniprot) Number Q61234
    Gene ID 20648
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat skeletal muscle membranes (lanes 2 and 4):
    1,2. Anti-α1-Syntrophin Antibody (#APZ-021), (1:400).
    3,4. Anti-α1-Syntrophin Antibody, preincubated with the control peptide antigen.
    Western blot analysis of mouse brain lysate:
    1. Anti-α1-Syntrophin Antibody (#APZ-021), (1:200).
    2. Anti-α1-Syntrophin Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of α1-Syntrophin in mouse neocortex.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-α1-Syntrophin Antibody (#APZ-021), (1:400), followed by anti-rabbit-AlexaFluor-488 antibody (green). α1-Syntrophin staining (green) appears in blood vessels (horizontal arrows) and in the pia mater (vertical arrows). Nuclei are stained with DAPI (blue).
    References
    1. Bhat, H.F. et al. (2014) Br. J. Cancer. 110, 706.
    2. Wilde, A.A. et al. (2011)  Circ. Res. 108, 884.
    3. Willis, B.C. et al. (2105)  Am. J. Physiol. Heart. Circ. Physiol. 308, 1463.
    4. Anderova, M. et al. (2014) PLoS One 9, e113444.
    5. Williams, J.C. et al. (006) J. Biol. Chem. 281, 23341.
    6. Koo, T. et al. (2011) Hum. Gene. Ther22, 1379.
    Scientific background

    α1-Syntrophin is a component of the dystrophin scaffold-protein complex that acts in recruiting proteins through their multiple protein–protein interaction motifs to the cytoplasmic side of plasma membranes. Proteins interacting with α1-syntrophin include voltage-gated sodium channels, neuronal nitric oxide synthase, calmodulin, ankyrin repeat-rich membrane spanning protein, G-proteins and more. 

    The syntrophin family comprises five members- (α1-syntrophin, β1-syntrophin, β2-syntrophin, γ1-syntrophin, and γ2-syntrophin1.

    α1- Syntrophin is a peripheral cytoplasmic membrane protein in skeletal and cardiac muscle cells1.

    α1- Syntrophin structure contains four conserved domains, two pleckstrin homology domains (PH1 and PH2), a PSD95-Disks large-ZO1 (PDZ) domain  and a syntrophin unique (SU) COOH-terminal domain2. The pleckstrin domains are responsible for the recruitment of proteins to the sarcolemma.  The PDZ domain is inserted within the PH1 domain and binds to NOS-1 in skeletal muscle. This domain also binds to the C-terminus of the Kir2.1 channel and also to NaV1.5 and so allowing them to interact. The SU domain binds syntrophin to dystrophin2,3. α1-Syntrophin is also responsible for the site-specific anchoring of AQP4 protein. α1-Syntrophin deletion leads to the disruption of the polarized subcellular expression of AQP4 in the perivascular membranes4

    Several studies have shown that syntrophin-deficiency in Duchenne muscular dystrophy can ultimately lead to muscle degeneration. Syntrophin-deficiency was also shown to reduce levels of nNOS in mice5,6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:
    Immuno-colocalization of Kir4.1 and α1-Syntrophin in rat fornix
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#AGP-012), (1:400) and rabbit Anti-α1-Syntrophin Antibody (#APZ-021), (1:300). A. Kir4.1 staining (red) appears in blood vessel profiles (arrows) in the fornix. B. α1-Syntrophin staining in the same section (green) appears in several elements including blood vessels (arrows). C. Merge of the two images reveals partial co-localization of Kir4.1 and α1-Syntrophin in hilar cells. Cell nuclei are stained with DAPI (blue).
    Last update: 29/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse α1-Syntrophin. Anti-α1-Syntrophin Antibody (#APZ-021) can be used in western blot and immunohistochemistry applications. It has been designed to recognize α1-Syntrophin from mouse, rat and human samples.

    For research purposes only, not for human use