Anti-α1-Syntrophin (SNTA1) Antibody

Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1
Cat #: APZ-021
Alternative Name Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RQPSSPGPQPRNLSE, corresponding to amino acid residues 191-205 of mouse α1-syntrophin (Accession Q61234). Intracellular, PH1 domain.
    Accession (Uniprot) Number Q61234
    Gene ID 20648
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
      • Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat skeletal muscle membranes (lanes 2 and 4):
        1,2. Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:400).
        3,4. Anti-α1-Syntrophin (SNTA1) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of mouse brain lysate:
        1. Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:200).
        2. Anti-α1-Syntrophin (SNTA1) Antibody, preincubated with the control peptide antigen.
      • Expression of Syntrophin alpha 1 in mouse neocortex
        Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:400), followed by anti-rabbit-AlexaFluor-488 antibody (green). Syntrophin alpha 1 staining (green) appears in blood vessels (horizontal arrows) and in the pia mater (vertical arrows). Nuclei are stained with DAPI (blue).
      • α1-Syntrophin is a component of the dystrophin scaffold-protein complex that acts in recruiting proteins through their multiple protein–protein interaction motifs to the cytoplasmic side of plasma membranes. Proteins interacting with α1-syntrophin include voltage-gated sodium channels, neuronal nitric oxide synthase, calmodulin, ankyrin repeat-rich membrane spanning protein, G-proteins and more. 

        The syntrophin family comprises five members: α1-syntrophin, β1-syntrophin, β2-syntrophin, γ1-syntrophin, and γ2-syntrophin1.

        α1-Syntrophin is a peripheral cytoplasmic membrane protein in skeletal and cardiac muscle cells1.

        The α1-syntrophin structure contains four conserved domains, two pleckstrin homology domains (PH1 and PH2), a PSD95-disks large-ZO1 (PDZ) domain and a syntrophin unique (SU) COOH-terminal domain2. The pleckstrin domains are responsible for the recruitment of proteins to the sarcolemma. The PDZ domain is inserted within the PH1 domain and binds to NOS-1 in skeletal muscle. This domain also binds to the C-terminus of the Kir2.1 channel and also to NaV1.5 and so allowing them to interact. The SU domain binds syntrophin to dystrophin2,3. α1-Syntrophin is also responsible for the site-specific anchoring of AQP4 protein. α1-Syntrophin deletion leads to the disruption of the polarized subcellular expression of AQP4 in the perivascular membranes4

        Several studies have shown that syntrophin-deficiency in Duchenne muscular dystrophy can ultimately lead to muscle degeneration. Syntrophin-deficiency was also shown to reduce levels of nNOS in mice5,6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:
    Anti-α1-Syntrophin (SNTA1) Antibody
    Immuno-colocalization of Kir4.1 and α1-Syntrophin in rat fornix
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#AGP-012), (1:400) and rabbit Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021), (1:300). A. Kir4.1 staining (red) appears in blood vessel profiles (arrows) in the fornix. B. Syntrophin alpha 1 staining in the same section (green) appears in several elements including blood vessels (arrows). C. Merge of the two images reveals partial co-localization of Kir4.1 and syntrophin alpha 1 in hilar cells. Cell nuclei are stained with DAPI (blue).
    Last update: 12/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse syntrophin alpha 1. Anti-α1-Syntrophin (SNTA1) Antibody (#APZ-021) can be used in western blot and immunohistochemistry applications. It has been designed to recognize syntrophin alpha 1 from mouse, rat and human samples.

    For research purposes only, not for human use