Anti-NaV1.5 (SCN5A) (493-511) Antibody

Sodium channel protein type 5 subunit alpha, SKM2
    Cat #: ASC-005
    Alternative Name Sodium channel protein type 5 subunit alpha, SKM2
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 of rat NaV1.5 (accession number P15389). Intracellular loop between domains I and II.
    • Anti-NaV1.5 (SCN5A) (493-511) Antibody
    Accession (Uniprot) Number P15389
    Gene ID 25665
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 17/19 amino acid residues identical.
    RRID AB_2040001.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-NaV1.5 (SCN5A) (493-511) Antibody
      Western blot analysis of rat heart membranes:
      1. Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200).   
      2. Anti-NaV1.5 (SCN5A) (493-511) Antibody, preincubated with the control peptide antigen.
    • Anti-NaV1.5 (SCN5A) (493-511) Antibody
      Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells:
      1. Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200).   
      2. Anti-NaV1.5 (SCN5A) (493-511) Antibody, preincubated with the control peptide antigen.
    • Mouse heart lysate (1:200) (Watanabe, H. et al. (2011) Circulation 124, 1001.).
    Immunoprecipitation
    • Mouse cardiac myocytes (1:500) (Malhotra, J.D. et al. (2001) Circulation 103, 1303.).
    Immunohistochemistry
    • Anti-NaV1.5 (SCN5A) (493-511) Antibody
      Expression of NaV1.5 in rat heart
      Immunohistochemical staining of NaV1.5 in rat myocardium paraffin-embedded sections using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the small vessels are negative (red arrows). Hematoxilin is used as the counterstain.
    • Anti-NaV1.5 (SCN5A) (493-511) Antibody
      Expression of NaV1.5 in mouse heart
      Immunohistochemical staining of mouse heart sections using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200). NaV1.5 staining (green) is part of the striate morphology. Nuclei are stained with DAPI (blue).
    Immunocytochemistry
    • Neonatal rat ventricular cardiomyocytes (NRCs) (1:200) (Casini, S. et al. (2010) Cardiovasc. Res. 85, 691.).
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029.
    Scientific background

    Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.

    The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.5.2

    The putative structure of NaV1.5 consists of four homologous domains (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome.1-2 Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system.1

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Nav1.5 (SCN5A) (493-511) AntibodyDecreased expression of NaV1.5 in RyR2s/s atria.Immunohistochemical staining of mouse atria using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), Anti-NaV1.6 (SCN8A) Antibody (#ASC-009) and Anti-SCN1A (NaV1.1) Antibody (#ASC-001). NaV1.5 staining in RyR2s/s atria is lower compared to wild type levels. NaV1.6 staining slightly decreased in RyR2s/s atria. NaV1.1 which is not expressed in the atrial tissue is not detected.Adapted from King, J.H. et al. (2013) with permission of Elsevier.
    Last update: 24/01/2020

    Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Immunohistochemical staining of mouse lumbar spinal cord sections. Tested in NaV1.5-/- mice.
      Pappalardo, L.W. et al. (2018) Glia 66, 2174.
    Western blot citations
    1. Rat brain neurolemma lysate.
      Murenzi, E. et al. (2017) Neurotoxicology 60, 260.
    2. Mouse neonatal ventricular myocytes.
      Xu, Q. et al. (2016) Am. J. Physiol. 311, H1139.
    3. Mouse heart lysate.
      Wang, N. et al. (2016) Free Radic. Biol. Med. 96, 34.
    4. Rat heart myoblast (H9C2) cell lysate.
      Baroni, D. et al. (2014) Biol. Cell 106, 13.
    5. Mouse muscle lysate.
      Boyer, J.G. et al. (2013) Skelet. Muscle 3, 24.
    6. Mouse heart lysate (1:200).
      Watanabe, H. et al. (2011) Circulation 124, 1001.
    Immunoprecipitation citations
    1. Mouse neonatal ventricular myocytes.
      Xu, Q. et al. (2016) Am. J. Physiol. 311, H1139.
    2. Mouse cardiac myocytes (1:500).
      Malhotra, J.D. et al. (2001) Circulation 103, 1303.
    Immunohistochemistry citations
    1. Mouse lumbar spinal cord sections. Also tested in NaV1.5-/- mice.
      Pappalardo, L.W. et al. (2018) Glia 66, 2174.
    2. Mouse heart sections.
      Chowdhury, R. et al. (2015) Circ. Arrythm. Electrophysiol. 8, 1255.
    Immunocytochemistry citations
    1. Human colon cancer (SW620) cells and HEK-293 transfected cells.
      Baptista-Hon, D.T. et al. (2014) Br. J. Anaesth. 113, i39.
    2. Transfected HEK-293 cells (1:300).
      Zumhagen, S. et al. (2013) PLoS ONE 8, e67963.
    3. Neonatal rat ventricular cardiomyocytes (NRCs) (1:200).
      Casini, S. et al. (2010) Cardiovasc. Res. 85, 691.
    More product citations
    1. Kim, J.J. et al. (2013) Am. J. Physiol. 304, H848.
    2. King, J.H. et al. (2013) Cardiovasc. Res. 99, 751.
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