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Anti-Aquaporin 1-ATTO Fluor-594 Antibody

AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Cat #: AQP-001-AR
Alternative Name AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1 (Accession P29972). Intracellular, C-terminus.
Accession (Uniprot) Number P29972
Gene ID 358
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, cattle, sheep - identical; dog - 18/19 amino acid residues identical; frog (Rana esculenta), toad (Bufo marinus) - 14/19 amino acid residues identical.
RRID AB_2341062.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AQP-001), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
  • Expression of AQP1 in rat kidney
    Expression of AQP1 in rat kidney
    Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1-ATTO Fluor-594 Antibody (#AQP-001-AR), (1:100). AQP1 staining (red) is present in convoluted tubules in the renal cortex. Glomeruli (G) are negative. Cell nuclei were visualized with Hoechst 33342 (blue).
References
  1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
  2. Ma, T. et al. (1998) J Biol Chem 273, 4296.
  3. Hu. J. and Verkman, A.S. (2006) FASEB J. 20, 1892.
Scientific background

Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2AQP-4 and AQP-5 belongs to the first group.1

Little is known about the function of the two newest members, AQP-11 and AQP-12.

The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1

AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration.2 In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-Aquaporin 1 Antibody (#AQP-001) is a highly specific antibody directed against an epitope of the human AQP1 channel. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP1 from rat, mouse, and human samples.

Anti-Aquaporin 1-ATTO Fluor-594 Antibody (#AQP-001-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 1-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
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