Free shipping starts now, no minimum, no coupons required!

Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody

WCH4, Mercurial-insensitive water channel

Cat #: AQP-014-AR
Alternative Name WCH4, Mercurial-insensitive water channel
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
  • Peptide (C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4 (Accession P47863). Intracellular, C-terminus.
Accession (Uniprot) Number P47863
Gene ID 25293
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.
RRID AB_2756786.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 – 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AQP-014), and immunohistochemical staining (labeled antibody).
Applications: if, ih
May also work in: ic*
  • Expression of Aquaporin 4 in rat and mouse brain
    Expression of Aquaporin 4 in rat and mouse brain
    Immunohistochemical staining of Aquaporin 4 in rat and mouse brain free floating frozen sections using Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (#AQP-014-AR), (1:80). A. AQP4 staining (red) in rat hippocampal CA1 region appears in outlines of blood vessels (arrows). DAPI is used as the counterstain (blue) and stains the pyramidal layer (P). B. AQP4 staining (red) in mouse hippocampal dentate gyrus region appears also in outlines of blood vessels (arrows). DAPI is used as the counterstain (blue) and stains the granule layer (G).
  • Multiplex staining of GFAP and AQP4 in rat brain
    Multiplex staining of GFAP and AQP4 in rat brain
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GFAP Antibody (#AFP-001), (1:2000) and Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (#AQP-014-AR), (1:100). A. In rat fornix, GFAP immunoreactivity (green) appears in astrocytic processes. B. In same section, AQP4 staining (red) is detected in blood vessels. C. Merge of panel A and panel B shows colocalization around a large blood vessel (arrows). Nuclei are stained using DAPI as the counterstain (blue).
  1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
  2. Manley, G.T. et al. (2004) Neuroscience. 129, 983.
  3. Lennon, V.A. et al. (2005) J. Exp. Med. 202, 473.
Scientific background

Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.

The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.

AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.

In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody
Multiplex staining of Aquaporin 4 and TrkB in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat cerebellum sections using Anti-TrkB (extracellular)-ATTO Fluor-488 Antibody (#ANT-019-AG), (1:80) and Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (#AQP-014-AR), (1:80). A. TrkB labeling (green) appears in Purkinje cells (arrows) forming the Purkinje layer (P). B. Aquaporin 4 staining (red) appears in outlines of the “baskets” formed around Purkinje cells (vertical arrows) and in some blood vessels (horizontal arrow). C. Merge of panels A and B demonstrates the localization of TrkB and Aquaporin 4 in adjacent cellular compartments of the cerebellum. Nuclei were demonstrated using DAPI as the counterstain (blue).
Last update: 12/08/2021

Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014) is a highly specific antibody directed against an epitope of rat AQP4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize the Aquaporin 4 channel from rat, mouse, and human samples.

Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (#AQP-014-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
Shipping and Ordering information