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Anti-Sigma-1 Receptor Antibody

SIGMAR1, OPRS1, Sigma non-opioid intracellular receptor 1, SIG-1R, Aging-associated gene 8 protein, SR31747-binding protein, SRBP
Cat #: AIP-006
Alternative Name SIGMAR1, OPRS1, Sigma non-opioid intracellular receptor 1, SIG-1R, Aging-associated gene 8 protein, SR31747-binding protein, SRBP
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SRGHSGRYWAEISD, corresponding to amino acid residues 113-126 of human SIGMAR1 (Accession Q99720). Intracellular, C-terminus.
Accession (Uniprot) Number Q99720
Gene ID 10280
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse – identical.
RRID AB_2756651.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) liver membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) liver membranes:
    1,2. Anti-Sigma-1 Receptor Antibody (AIP-006), (1:200).
    3,4. Anti-Sigma-1 Receptor Antibody, preincubated with Sigma-1 Receptor Blocking Peptide (#BLP-IP006).
References
  1. Mansour, A. et al. (1995) Trends. Neurosci. 18, 22.
  2. Jin, J.L. et al. (2015) Int. J. Clin. Exp. Med. 8, 4808.
Scientific background

Sigma receptors were first identified as a subtype of opioid receptors. The different members of the family are differentially distributed in various parts of the CNS and are responsible for a vast range of functions and behaviors1. Today, sigma receptors are mostly considered to be a separate receptor family with two main subtypes characterized.

The sigma-1 receptor is encoded by a gene baring the same name and is a protein of 223 amino acids with two transmembrane domains and a typical endoplasmic reticulum localized signal near its short N terminus. Sigma receptors are mostly detected in the central nervous system with a high density in the spinal cord, pons, medulla oblongata, red nucleus, cerebellum and hippocampus.

Sigma-1 is mainly localized on the mitochondrial associated endoplasmic reticulum membrane (MAM), forming a BiP chaperone structure with high sensitivity to the calcium ion. When activated by agonists such as cocaine or analgesics, sigma-1 receptors separate from BiP and translocate from MAM to other parts of the cell. Through regulation of inositol triphosphate (IP3) receptorsNMDA receptorsdopamine receptors and voltage-gated Ca2+ channels, sigma-1 receptors can influence TCA cycle, oxidative stress, mitochondrial function, neuron plasticity and the release of various neurotransmitters.

The exact etiology and pathophysiology of Alzheimer’s disease is not fully understood. A few hypotheses suggest the involvement of Sigma-1 in the progression of Alzheimer’s disease. The suggested mechanisms include protection from Aβ toxicity, prevention of Tau protein hyperphosphorylation and the upregulation of acetylcholine release2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Sigma-1 Receptor Antibody (#AIP-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SIGMAR1 from human, rat, and mouse samples.

For research purposes only, not for human use
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