- Peptide CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat CaV3.3 (Accession number Q9Z0Y8). Intracellular, between domains II and III.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400).
3-4. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).
- Mouse hindbrain (Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.).
- Expression of CaV3.3 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400), followed by donkey anti-rabbit-Cy3. A. Staining in the hippocampal CA3 region, showed Cav3.3 immunoreactivity (red) appeared in soma of pyramidal neuron profiles (horizontal arrows) and in dendrites (vertical arrows) projecting into the stratum radiatum (SR). B. Pre-incubation of the antibody with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.
- Human sperm cells (1:50) (Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.).
- Park, J.Y. et al. (2004) J. Biol. Chem. 279, 21707.
- Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678.
- Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530.
- Gomora, J.C. et al. (2002) Biophys. J. 83, 229.
T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation.
T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.1 (CACNA1G), CaV3.2 (CACNA1H) and CaV3.3 (CACNA1I).1-3
While CaV3.1 and CaV3.2 are widely expressed in various tissues, CaV3.3 is primarily expressed in the central nervous system, where high expression has been described in thalamic neurons.
The Ca2+ current generated by the CaV3.3 channel displays much slower activation and inactivation compared to the currents produced by CaV3.1 and CaV3.2, suggesting it might play a different role in neuronal excitability.1,4
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the CaV3.3 channel. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009) can be used in western blot, immunohistochemical and immunocytochemical applications. It has been designed to recognize CaV3.3 from rat, human and mouse samples.
- Human artery lysates (1:200).
Harraz, O.F. et al. (2015) J. Gen. Physiol. 145, 405.
- Mouse hindbrain.
Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.
- Human sperm cells (1:50).
Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.
- Weiss, N. et al. (2012) J. Biol. Chem. 287, 2810.
- Anderson, D. et al. (2010) Nat. Neurosci. 13, 333.
- Kovács, K. et al. (2010) J. Neurosci. Res. 88, 448.