- Peptide CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat CaV3.3 (Accession number Q9Z0Y8). Intracellular, between domains II and III.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400).
3-4. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).
- Mouse hindbrain (Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.).
- Human sperm cells (1:50) (Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.).
T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation.
T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.1 (CACNA1G), CaV3.2 (CACNA1H) and CaV3.3 (CACNA1I).1-3
While CaV3.1 and CaV3.2 are widely expressed in various tissues, CaV3.3 is primarily expressed in the central nervous system, where high expression has been described in thalamic neurons.
The Ca2+ current generated by the CaV3.3 channel displays much slower activation and inactivation compared to the currents produced by CaV3.1 and CaV3.2, suggesting it might play a different role in neuronal excitability.1,4