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Alomone Labs TTA-P2 inhibits T-type CaV channels heterologously expressed in Xenopus oocytes.A. Time course of CaV3.2 peak current amplitude, elicited by 100 ms voltage step from holding potential of -100mV to -30 mV, delivered every 10 seconds. Application of 5 µM TTA-P2 (#T-155) inhibits the CaV3.2 current in a reversible manner (indicated by the horizontal bar). B. Representative current traces before and during application of 5 µM TTA-P2 as indicated.
TTA-P2 is a novel, potent and selective T-type CaV blocker. In thalamocortical and reticular neurons, it blocks T-type currents with an IC50 of 22 nM without affecting high voltage Ca2+ channels1. In rat DRGs, it blocks T-type currents with an IC50 of 100 nM and stabilizes the channel in the inactive state2.
TTA-P2 has the potential of acting as an antinociceptive agent2.
Alomone Labs TTA-P2 inhibits T-type CaV channels in AZF cells.Bovine adrenal zona fasciculate (AZF) whole cell recordings. Ca2+ currents were recorded in 10 mM Ba2+ in response to voltage steps to -5 mV, applied from a holding potential of -80 mV, before and after superfusion of the cell with 2 μM TTA-P2 (#T-155), (left panel). Concentration-dependent inhibition of CaV3.2 currents by TTA-P2. Values are means ± SE for number of determinations shown in parentheses (right panel).Adapted from Enyeart, J.J. and Enyeart, J.A. (2015) Am. J. Physiol. 308, C899. with permission of the American Physiological Society.
TTA-P2 (#T-155) is a highly pure, synthetic, and biologically active compound.
- Rat DRGs (patch clamp).
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- Rat DRGs (patch clamp).
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