Anti-CaV3.3 (CACNA1I) Antibody

Voltage-dependent T-type calcium channel subunit α1I
    Cat #: ACC-009
    Alternative Name Voltage-dependent T-type calcium channel subunit α1I
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat CaV3.3 (Accession number Q9Z0Y8). Intracellular, between domains II and III.
    • Anti-CaV3.3 (CACNA1I) Antibody
    Accession (Uniprot) Number Q9Z0Y8
    Gene ID 56827
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - 14/15 amino acid residues identical.
    RRID AB_2039783.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-CaV3.3 (CACNA1I) Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:200).  
      2. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Mouse hindbrain (Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.).
    Immunocytochemistry
    • Human sperm cells (1:50) (Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.).
    References
    1. Park, J.Y. et al. (2004) J. Biol. Chem. 279, 21707.
    2. Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678.
    3. Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530.
    4. Gomora, J.C. et al. (2002) Biophys. J. 83, 229.
    Scientific background

    T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation.  

    T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.1 (CACNA1G), CaV3.2 (CACNA1H) and CaV3.3 (CACNA1I).1-3  

    While CaV3.1 and CaV3.2 are widely expressed in various tissues, CaV3.3 is primarily expressed in the central nervous system, where high expression has been described in thalamic neurons.  

    The Ca2+ current generated by the CaV3.3 channel displays much slower activation and inactivation compared to the currents produced by CaV3.1 and CaV3.2, suggesting it might play a different role in neuronal excitability.1,4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the CaV3.3 channel. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009) can be used in western blot, immunohistochemical and immunocytochemical applications. It has been designed to recognize CaV3.3 from rat, human and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Human artery lysates (1:200).
      Harraz, O.F. et al. (2015) J. Gen. Physiol. 145, 405.
    Immunohistochemistry citations
    1. Mouse hindbrain.
      Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.
    Immunocytochemistry citations
    1. Human sperm cells (1:50).
      Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.
    More product citations
    1. Weiss, N. et al. (2012) J. Biol. Chem. 287, 2810.
    2. Anderson, D. et al. (2010) Nat. Neurosci. 13, 333.
    3. Kovács, K. et al. (2010) J. Neurosci. Res. 88, 448.
    Shipping and Ordering information