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Anti-CaV3.3 (CACNA1I) Antibody

Voltage-dependent T-type calcium channel subunit α1I
Cat #: ACC-009
Alternative Name Voltage-dependent T-type calcium channel subunit α1I
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat CaV3.3 (Accession number Q9Z0Y8). Intracellular, between domains II and III.
Accession (Uniprot) Number Q9Z0Y8
Gene ID 56827
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - 14/15 amino acid residues identical.
RRID AB_2039783.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.75 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:200).  
    2. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).
Immunohistochemistry
  • Mouse hindbrain (Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.).
Immunocytochemistry
  • Human sperm cells (1:50) (Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.).
References
  1. Park, J.Y. et al. (2004) J. Biol. Chem. 279, 21707.
  2. Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678.
  3. Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530.
  4. Gomora, J.C. et al. (2002) Biophys. J. 83, 229.
Scientific background

T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation.  

T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.1 (CACNA1G), CaV3.2 (CACNA1H) and CaV3.3 (CACNA1I).1-3  

While CaV3.1 and CaV3.2 are widely expressed in various tissues, CaV3.3 is primarily expressed in the central nervous system, where high expression has been described in thalamic neurons.  

The Ca2+ current generated by the CaV3.3 channel displays much slower activation and inactivation compared to the currents produced by CaV3.1 and CaV3.2, suggesting it might play a different role in neuronal excitability.1,4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the CaV3.3 channel. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009) can be used in western blot, immunohistochemical and immunocytochemical applications. It has been designed to recognize CaV3.3 from rat, human and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Human artery lysates (1:200).
    Harraz, O.F. et al. (2015) J. Gen. Physiol. 145, 405.
Immunohistochemistry citations
  1. Mouse hindbrain.
    Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.
Immunocytochemistry citations
  1. Human sperm cells (1:50).
    Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.
More product citations
  1. Weiss, N. et al. (2012) J. Biol. Chem. 287, 2810.
  2. Anderson, D. et al. (2010) Nat. Neurosci. 13, 333.
  3. Kovács, K. et al. (2010) J. Neurosci. Res. 88, 448.
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